Price range: $268.00 through $328.00
Background:
This gene encodes a subunit of the anaphase-promoting complex. This complex is an E3 ubiquitin ligase that regulates progression through the metaphase to anaphase portion of the cell cycle by ubiquitinating proteins which targets them for degradation. [provided by RefSeq, Dec 2011],
| Size | 50μL/100μL |
| Target | APC1 |
| Reactivity | Human, Mouse, Rat |
| Applications | IHC, IF, ELISA |
| MW(Calculated) | 217kD |
| MW(Observed) | |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human APC1. AA range:654-703 |
| Specificity | APC1 Polyclonal Antibody detects endogenous levels of APC1 protein. |
| Gene Name | ANAPC1 |
| Protein Name | Anaphase-promoting complex subunit 1 |
| Other Name | ANAPC1; / TSG24; / Anaphase-promoting complex subunit 1; / APC1; / Cyclosome subunit 1; / Mitotic checkpoint regulator; / Testis-specific gene 24 protein |
| Species | Human |
| Gene ID | 64682 |
| Uniprot | Q9H1A4, |
| Species.1 | Mouse |
| Gene ID.1 | 17222 |
| Uniprot.1 | P53995 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | nucleoplasm,anaphase-promoting complex,cytosol, |
| Tissue Expression | Colon,Epithelium,Ovary,Placenta, |
| Signaling_pathway | Cellular Processes >> Cell growth and death >> Cell cycle |
| Research Areas | >>Cell cycle; / >>Oocyte meiosis; / >>Ubiquitin mediated proteolysis; / >>Progesterone-mediated oocyte maturation; / >>Human T-cell leukemia virus 1 infection |
| Function | Function:Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle.,pathway:Protein modification; protein ubiquitination.,PTM:Phosphorylated. Phosphorylation on Ser-355 occurs specifically during mitosis.,similarity:Belongs to the APC1 family.,similarity:Contains 4 PC repeats.,subunit:The APC/C is composed of at least 11 subunits., |
