Apelin Polyclonal Antibody
Ucallm_WP15243-50
Price range: $268.00 through $328.00
Product details
Background:
This gene encodes a peptide that functions as an endogenous ligand for the G-protein coupled apelin receptor. The encoded preproprotein is proteolytically processed into biologically active C-terminal peptide fragments. These peptide fragments activate different tissue specific signaling pathways that regulate diverse biological functions including fluid homeostasis, cardiovascular function and insulin secretion. This protein also functions as a coreceptor for the human immunodeficiency virus 1. [provided by RefSeq, Feb 2016],
Specifications
| Size | 50μL/100μL |
| Target | Apelin |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 16kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC: 1:100-300; ELISA 1:20000; IF 1:100-300; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from the C-terminal region of human APLN. AA range:28-77 |
| Specificity | Apelin Polyclonal Antibody detects endogenous levels of Apelin protein. |
| Gene Name | APLN |
| Protein Name | Apelin |
| Other Name | APLN; / APEL; / Apelin; / APJ endogenous ligand |
| Species | Human |
| Gene ID | 8862 |
| Uniprot | Q9ULZ1, |
| Species.1 | Mouse |
| Gene ID.1 | 30878 |
| Uniprot.1 | Q9R0R4, |
| Species.2 | Rat |
| Gene ID.2 | 58812 |
| Uniprot.2 | Q9R0R3 |
| Cellular Localization | Secreted . Secreted, extracellular space . Abundantly secreted in the colostrum. Lower level in milk. Decreases rapidly within several days after parturition in milk, but is still detectable even in commercial milk. . |
| Tissue Expression | Expressed in the brain with highest levels in the frontal cortex, thalamus, hypothalamus and midbrain (PubMed:10617103). Secreted by the mammary gland into the colostrum and the milk. |
| Signaling_pathway | Environmental Information Processing >> Signal transduction >> Apelin signaling pathway |
| Research Areas | >>Neuroactive ligand-receptor interaction; / >>Apelin signaling pathway |
| Function | Function:Endogenous ligand for APJ, an alternative coreceptor with CD4 for HIV-1 infection. Inhibits HIV-1 entry in cells coexpressing CD4 and APJ. Apelin-36 has a greater inhibitory activity on HIV infection than other synthetic apelin derivatives. The oral intake in the colostrum and the milk could have a role in the modulation of the immune responses in neonates. May also have a role in the central control of body fluid homeostasis by influencing AVP release and drinking behavior.,PTM:Several active peptides may be produced by proteolytic processing of the peptide precursor.,similarity:Belongs to the apelin family.,tissue specificity:Expressed in the brain with highest levels in the frontal cortex, thalamus, hypothalamus and midbrain. Secreted by the mammary gland into the colostrum and the milk., |
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