SKU: WM1004 Categories: ,

Aquaporin 4 Monoclonal Antibody [4H1]

Price range: $268.00 through $328.00

SKU: WM1004-50
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Product details

Background:
This gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. This protein is the predominant aquaporin found in brain and has an important role in brain water homeostasis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. A recent study provided evidence for translational readthrough in this gene and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon. [provided by RefSeq, Dec 2015],

Specifications

Size50μL/100μL
TargetAQP4
ReactivityHuman, Mouse, Rat
ApplicationsWB, IHC, IF
MW(Calculated)
MW(Observed)48kD
Host SpeciesMouse
Isotype
Conjugate/ModificationUnmodified
Recommended Dilution RatioWB 1:1000; IF 1:100-200; IHC 1:50-300 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationPBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
PurificationThe antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
ClonalityMonoclonal
Clone Number4H1
ImmunogenSynthetic Peptide of Aquaporin 4
SpecificityThe antibody detects endogenous Aquaporin 4 proteins.
Gene NameAQP4
Protein NameAquaporin-4
Other NameAQP4; / Aquaporin-4; / AQP-4; / Mercurial-insensitive water channel; / MIWC; / WCH4
SpeciesHuman
Gene ID361
UniprotP55087,
Species.1Mouse
Gene ID.111829
Uniprot.1P55088,
Species.2Rat
Gene ID.225293
Uniprot.2P47863
Cellular LocalizationCell membrane; Multi-pass membrane protein . Basolateral cell membrane; Multi-pass membrane protein . Endosome membrane . Cell membrane, sarcolemma; Multi-pass membrane protein . Cell projection . Activation of the vasopressin receptor AVPR1A triggers AQP4 phosphorylation at Ser-180 and promotes its internalization from the cell membrane. Detected on brain astrocyte processes and astrocyte endfeet close to capillaries. .
Tissue ExpressionDetected in skeletal muscle (PubMed:29055082). Detected in stomach, along the glandular base region of the fundic gland (at protein level) (PubMed:8601457). Detected in brain, lung and skeletal muscle, and at much lower levels in heart and ovary (PubMed:7559426, PubMed:8601457).
Signaling_pathway
Research Areas>>Vasopressin-regulated water reabsorption; / >>Bile secretion
FunctionDomain:Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).,Function:Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system.,similarity:Belongs to the MIP/aquaporin (TC 1.A.8) family.,tissue specificity:Brain - muscle >> heart, kidney, lung, and trachea.,
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