Name: ARF6 Polyclonal Antibody
SKU: WP11111
Price: 268 USD
Availability: InStock

Product details

Background:

ADP ribosylation factor 6(ARF6) Homo sapiens This gene encodes a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of this gene is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. A pseudogene of this gene is located on chromosome 7. [provided by RefSeq, Jul 2008],

Specifications

Size	50μL/100μL
Target	ARF6
Reactivity	Human, Mouse, Rat
Applications	WB, ELISA
MW(Calculated)
MW(Observed)	20kD
Host Species	Rabbit
Isotype	IgG
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:500-1:2000; ELISA 1:10000; Not yet tested in other applications.
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration	1 mg/ml
Clonality	Polyclonal
Clone Number
Immunogen	Synthesized peptide derived from ARF6 . at AA range: 30-110
Specificity	ARF6 Polyclonal Antibody detects endogenous levels of ARF6 protein.
Gene Name	ARF6
Protein Name	ADP-ribosylation factor 6
Other Name	ARF6; / ADP-ribosylation factor 6
Species	Human
Gene ID	382
Uniprot	P62330,
Species.1	Mouse
Gene ID.1	11845
Uniprot.1	P62331,
Species.2	Rat
Gene ID.2	79121
Uniprot.2	P62332
Cellular Localization	Cytoplasm, cytosol . Cell membrane; Lipid-anchor . Endosome membrane; Lipid-anchor . Recycling endosome membrane; Lipid-anchor . Cell projection, filopodium membrane; Lipid-anchor . Cell projection, ruffle . Cleavage furrow . Midbody, Midbody ring . Early endosome membrane; Lipid-anchor . Golgi apparatus, trans-Golgi network membrane; Lipid-anchor . Distributed uniformly on the plasma membrane, as well as throughout the cytoplasm during metaphase. Subsequently concentrated at patches in the equatorial region at the onset of cytokinesis, and becomes distributed in the equatorial region concurrent with cleavage furrow ingression. In late stages of cytokinesis, concentrates at the midbody ring/Flemming body (PubMed:23603394). Recruitment to the midbody ring requires both activation by PSD/EFA6A and interaction with KIF23/MKLP1 (PubMed:23603394). After abscission of the intercellular bridge, incorporated into one of the daughter cells as a midbody remnant and localizes to punctate structures beneath the plasma membrane (PubMed:23603394). Recruited to the cell membrane in association with CYTH2 and ARL4C (PubMed:17398095). Colocalizes with DAB2IP at the plasma membrane and endocytic vesicles (PubMed:19948740). Myristoylation is required for proper localization to membranes: myristoylation on Lys-3 allows ARF6 to remain on membranes during the GTPase cycle (PubMed:7589240, PubMed:32103017). .
Tissue Expression	Ubiquitous, with higher levels in heart, substantia nigra, and kidney.
Signaling_pathway	Organismal Systems >> Immune system >> Fc gamma R-mediated phagocytosis;Environmental Information Processing >> Signal transduction >> Ras signaling pathway;Environmental Information Processing >> Signal transduction >> Phospholipase D signaling pathway
Research Areas	>>Ras signaling pathway; / >>Phospholipase D signaling pathway; / >>Endocytosis; / >>Fc gamma R-mediated phagocytosis; / >>Pathogenic Escherichia coli infection; / >>Shigellosis; / >>Salmonella infection; / >>Yersinia infection
Function	Function:GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.,similarity:Belongs to the small GTPase superfamily.,similarity:Belongs to the small GTPase superfamily. Arf family.,subunit:Interacts with ARHGAP21, ASAP2, HERC1, PIP5K1C and UACA.,

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ARF6 Polyclonal Antibody

Product details

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ARF6 Polyclonal Antibody

Product details

Specifications

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