Arkadia Polyclonal Antibody
Ucallm_WP11129-50
Price range: $268.00 through $328.00
Product details
Background:
ring finger protein 111(RNF111) Homo sapiens The protein encoded by this gene is a nuclear RING-domain containing E3 ubiquitin ligase. This protein interacts with the transforming growth factor (TGF) -beta/NODAL signaling pathway by promoting the ubiquitination and proteosomal degradation of negative regulators, like SMAD proteins, and thereby enhances TGF-beta target-gene transcription. As a modulator of the nodal signaling cascade, this gene plays a critical role in the induction of mesoderm during embryonic development. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2012],
Specifications
| Size | 50μL/100μL |
| Target | Arkadia |
| Reactivity | Human, Mouse |
| Applications | WB, ELISA |
| MW(Calculated) | |
| MW(Observed) | 110kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; ELISA 1:40000; Not yet tested in other applications. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human RNF111. AA range:901-950 |
| Specificity | Arkadia Polyclonal Antibody detects endogenous levels of Arkadia protein. |
| Gene Name | RNF111 |
| Protein Name | E3 ubiquitin-protein ligase Arkadia |
| Other Name | RNF111; / E3 ubiquitin-protein ligase Arkadia; / RING finger protein 111 |
| Species | Human |
| Gene ID | 54778 |
| Uniprot | Q6ZNA4, |
| Species.1 | Mouse |
| Gene ID.1 | 93836 |
| Uniprot.1 | Q99ML9 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus . Cytoplasm . Nucleus, PML body . Upon TGF-beta treatment, translocates from nucleus to cytosol. . |
| Tissue Expression | Broadly expressed. |
| Signaling_pathway | |
| Research Areas | |
| Function | Function:Acts in the NODAL pathway of mesoderm patterning during embryonic development. Acts downstream AXIN1 as an E3 ubiquitin-protein ligase which promotes the ubiquitination of inhibitory SMADs such as SMAD7, induces their proteasomal degradation and thereby enhances the transcriptional activity of TGF-beta and BMP. Activates Smad3/Smad4-dependent transcription by triggering signal-induced SnoN degradation.,pathway:Protein modification; protein ubiquitination.,similarity:Contains 1 RING-type zinc finger.,subcellular location:Upon TGF-beta treatment, translocates from nucleus to cytosol.,subunit:Interacts with SMAD6, SMAD7, AXIN1, AXIN2 and SKIL isoform SNON. Part of a complex containing RNF111, AXIN1 and SMAD7.,tissue specificity:Broadly expressed., |
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