Name: ASK1 (Phospho Thr845) rabbit pAb
SKU: WP10292
Price: 288 USD
Availability: InStock

Product details

Background:

Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1, 374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK. [provided by Re

Specifications

Target	ASK1 Phospho Thr845
Reactivity	Human, Mouse, Rat
Applications	WB
MW(Calculated)
MW(Observed)	155kD
Host Species	Rabbit
Isotype	IgG
Conjugate/Modification	phosphate
Modification
Recommended Dilution Ratio	WB 1:1000-2000
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Purification	The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Purity
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration	1 mg/ml
Clonality	Polyclonal
Clone Number
Immunogen	Synthesized phosho peptide around human ASK1 (Thr845)
Sequence
Specificity	This antibody detects endogenous levels of Human ASK1 (phospho-Thr845)
Gene Name	MAP3K5 ASK1 MAPKKK5 MEKK5
Protein Name	ASK1 (Thr845)
Other Name	Mitogen-activated protein kinase kinase kinase 5; / Apoptosis signal-regulating kinase 1; / ASK-1; / MAPK/ERK kinase kinase 5; / MEK kinase 5; / MEKK 5
Species	Human
Gene ID-1	4217
Uniprot	Q99683,
Species.1	Mouse
Gene ID-2	26408
Uniprot.1	O35099
Species.2
Gene ID-3
Uniprot.2
Organism-4
Gene ID-4
SwissProt-4
Background	Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK. [provided by Re
Cellular Localization	Cytoplasm . Endoplasmic reticulum. Interaction with 14-3-3 proteins alters the distribution of MAP3K5/ASK1 and restricts it to the perinuclear endoplasmic reticulum region.
Tissue Expression	Abundantly expressed in heart and pancreas.
Signaling_pathway	Cellular Processes >> Cell growth and death >> Apoptosis;Cellular Processes >> Cellular community - eukaryotes >> Tight junction;Organismal Systems >> Nervous system >> Neurotrophin signaling pathway;Human Diseases >> Neurodegenerative disease >> Alzheimer disease;Human Diseases >> Neurodegenerative disease >> Parkinson disease;Human Diseases >> Neurodegenerative disease >> Amyotrophic lateral sclerosis;Human Diseases >> Neurodegenerative disease >> Huntington disease;Human Diseases >> Neurodegenerative disease >> Spinocerebellar ataxia;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway;Environmental Information Processing >> Signal transduction >> TNF signaling pathway;Environmental Information Processing >> Signal transduction >> Sphingolipid signaling pathway
Research Areas	>>Platinum drug resistance; / >>MAPK signaling pathway; / >>Sphingolipid signaling pathway; / >>Protein processing in endoplasmic reticulum; / >>Apoptosis; / >>Tight junction; / >>TNF signaling pathway; / >>Thermogenesis; / >>Neurotrophin signaling pathway; / >>Non-alcoholic fatty liver disease; / >>Alcoholic liver disease; / >>Alzheimer disease; / >>Parkinson disease; / >>Amyotrophic lateral sclerosis; / >>Huntington disease; / >>Spinocerebellar ataxia; / >>Pathways of neurodegeneration - multiple diseases; / >>Chemical carcinogenesis - reactive oxygen species; / >>Lipid and atherosclerosis; / >>Fluid shear stress and atherosclerosis
Function	Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Contains an N-terminal autoinhibitory domain. Activated by phosphorylation at Thr-838, inhibited by phosphorylation at Ser-966 and Ser-1033. Binds to, and stabilizes MAP3K6 and is activated by MAP3K6 by phosphorylation on Thr-838.,Function:Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death.,induction:By TNF-alpha. Inhibited by HIV-1 Nef.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Homodimer when inactive. Binds both upstream activators and downstream substrates in multimolecular complexes. Associates with and inhibited by HIV-1 Nef. Interacts with DAB2IP and PPM1L.,tissue specificity:Abundantly expressed in heart and pancreas.,
RRID

Please login to post questions

ASK1 (Phospho Thr845) rabbit pAb

Product details

Specifications

About Us

Support

Order

Menu

ASK1 (Phospho Thr845) rabbit pAb

Product details

Specifications

Related products

U-Blot® CDK6 Rabbit mAb

U-Blot® Glutamine Synthetase Rabbit mAb

Acetyl Lysine Monoclonal Antibody [10B10]

U-Blot® VEGF Receptor 1 Rabbit mAb

U-Blot® CD86 Rabbit mAb

U-Blot® Ezrin Rabbit mAb

Customer Reviews

Menu