U-Blot® ATG3 Rabbit mAb
WR4238 Western blot analysis of lysates from Hela cells, using PP2A α/β Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.
Price range: $268.00 through $328.00
Product details
Background:This gene encodes a ubiquitin-like-conjugating enzyme and is a component of ubiquitination-like systems involved in autophagy, the process of degradation, turnover and recycling of cytoplasmic constituents in eukaryotic cells. This protein is known to play a role in regulation of autophagy during cell death. A pseudogene of this gene is located on chromosome 20. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013],
Specifications
| Size | 50μL/100μL |
| Target | ATG3 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 36kD |
| MW(Observed) | 36kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0538R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | ATG3 APG3 APG3L |
| Protein Name | ATG3 |
| Other Name | PP2A A; / PP2A alpha; / PP2A B; / PP2A beta; / PP2A-alpha; / PP2A-beta; / PP2AA_HUMAN; / PP2Aalpha; / PP2AB_HUMAN; / PP2Abeta; / PP2Ac; / PP2CA; / PP2Calpha; / PP2CB; / PPP2CA; / PPP2CB; / Protein phosphatase 2 catalytic subunit alpha isoform; / Protein phosphatase 2 catalytic subunit beta isoform; / Protein phosphatase type 2A catalytic subunit; / Replication protein C; / RP C; / RP-C; / RPC; / Serine/threonine protein phosphatase 2A catalytic subunit alpha isoform; / Serine/threonine protein phosphatase 2A catalytic subunit beta isoform; / Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform |
| Species | Human |
| Gene ID | 5515;5516 |
| Uniprot | Q9NT62, |
| Species.1 | Mouse |
| Gene ID.1 | 67841 |
| Uniprot.1 | Q9CPX6, |
| Species.2 | Rat |
| Gene ID.2 | 24672;24673 |
| Uniprot.2 | P63331;P62716 |
| Cellular Localization | Cytoplasm |
| Tissue Expression | Fibroblast,Liver,Lung,Placenta,Testis,Uterus,baseline |
| Signaling_pathway | Cellular Processes >> Transport and catabolism >> Autophagy - animal;Cellular Processes >> Cell growth and death >> Cell cycle;Cellular Processes >> Cellular community - eukaryotes >> Tight junction;Organismal Systems >> Immune system >> T cell receptor signaling pathway;Organismal Systems >> Circulatory system >> Adrenergic signaling in cardiomyocytes;Organismal Systems >> Nervous system >> Dopaminergic synapse;Environmental Information Processing >> Signal transduction >> TGF-beta signaling pathway;Environmental Information Processing >> Signal transduction >> Hippo signaling pathway;Environmental Information Processing >> Signal transduction >> Sphingolipid signaling pathway;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway;Environmental Information Processing >> Signal transduction >> AMPK signaling pathway |
| Research Areas | >>mRNA surveillance pathway; / >>Sphingolipid signaling pathway; / >>Oocyte meiosis; / >>Autophagy - other; / >>Autophagy - animal; / >>PI3K-Akt signaling pathway; / >>AMPK signaling pathway; / >>Adrenergic signaling in cardiomyocytes; / >>TGF-beta signaling pathway; / >>Hippo signaling pathway; / >>Tight junction; / >>Dopaminergic synapse; / >>Long-term depression; / >>Chagas disease; / >>Hepatitis C; / >>Human papillomavirus infection |
| Function | Function:GABARAPL1 (GABARAPL2 or GABARAP or MAP1LC3)-modifier protein conjugating enzyme involved in its E2-like covalent binding to PE. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3 (E2-like enzyme). Preferred substrate is MAP1LC3A. Formation of the GABARAPL1-PE conjugate is essential for autophagy.,similarity:Belongs to the ATG3 family.,subunit:Interacts with ATG7 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5.,tissue specificity:Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta., |
Please login to post questions
Related products
