Name: ATG5 [PTR1362] mouse mAb
SKU: WM1211
Price: 268 USD
Availability: InStock

Product details

Background:
The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Sep 2015],

Specifications

Target	ATG5
Reactivity	Human, Mouse
Applications	WB, IHC, IF, IP, ELISA
MW(Calculated)	32kD
MW(Observed)	55kD
Host Species	Mouse
Isotype	IgG
Conjugate/Modification	Unmodified
Modification
Recommended Dilution Ratio	IHC 1:200-1:1000; WB 1:500-2000; IF 1:100-500; ELISA 1:1000-5000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Source
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein G affinity chromatography.
Purity
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration	1 mg/ml
Clonality	Monoclonal
Clone Number	PTR1362
Immunogen	Recombinant Protein
Sequence
Specificity	This antibody detects endogenous levels of ATG5.
Gene Name	ATG5 APG5L ASP
Protein Name	Autophagy protein 5 (APG5-like) (Apoptosis-specific protein)
Other Name	Autophagy protein 5; / APG5-like; / Apoptosis-specific protein
Species	Human
Gene ID-1	9474
Uniprot	Q9H1Y0,
Species.1	Mouse
Gene ID-2
Uniprot.1	Q99J83,
Species.2	Rat
Gene ID-3
Uniprot.2	Q3MQ06
Organism-4
Gene ID-4
SwissProt-4
Background	The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Sep 2015],
Cellular Localization
Tissue Expression	Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells.
Signaling_pathway	Cellular Processes >> Transport and catabolism >> Autophagy - animal;Cellular Processes >> Cell growth and death >> Ferroptosis;Organismal Systems >> Immune system >> NOD-like receptor signaling pathway;Organismal Systems >> Immune system >> RIG-I-like receptor signaling pathway;Organismal Systems >> Aging >> Longevity regulating pathway;Organismal Systems >> Aging >> Longevity regulating pathway - multiple species
Research Areas	>>Autophagy - other; / >>Mitophagy - animal; / >>Autophagy - animal; / >>Longevity regulating pathway; / >>Longevity regulating pathway - multiple species; / >>Ferroptosis; / >>NOD-like receptor signaling pathway; / >>RIG-I-like receptor signaling pathway; / >>Shigellosis
Function	Function:May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.,Function:Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed.,induction:By apoptotic stimuli.,PTM:Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane.,similarity:Belongs to the ATG5 family.,subcellular location:Colocalizes with nonmuscle actin.,tissue specificity:Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells.,
RRID

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ATG5 [PTR1362] mouse mAb

Product details

Specifications

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ATG5 [PTR1362] mouse mAb

Product details

Specifications

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