U-Blot® ATG5 Rabbit mAb
WR4313 A549 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with primary antibody(1:1000). The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.
Price range: $268.00 through $328.00
Product details
Background:The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Sep 2015],
Specifications
| Size | 50μL/100μL |
| Target | ATG5 |
| Reactivity | Human, Mouse, Rat, Chicken |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 33kD |
| MW(Observed) | 55kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0514R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | ATG5 APG5L ASP |
| Protein Name | ATG5 |
| Other Name | Autophagy protein 5 (APG5-like; / Apoptosis-specific protein) |
| Species | Human |
| Gene ID | 9474 |
| Uniprot | Q9H1Y0, |
| Species.1 | Mouse |
| Gene ID.1 | 11793 |
| Uniprot.1 | Q99J83, |
| Species.2 | Rat |
| Gene ID.2 | |
| Uniprot.2 | Q3MQ06 |
| Cellular Localization | Cytoplasm |
| Tissue Expression | Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells. |
| Signaling_pathway | Cellular Processes >> Transport and catabolism >> Autophagy - animal;Cellular Processes >> Cell growth and death >> Ferroptosis;Organismal Systems >> Immune system >> NOD-like receptor signaling pathway;Organismal Systems >> Immune system >> RIG-I-like receptor signaling pathway;Organismal Systems >> Aging >> Longevity regulating pathway;Organismal Systems >> Aging >> Longevity regulating pathway - multiple species |
| Research Areas | >>Autophagy - other; / >>Mitophagy - animal; / >>Autophagy - animal; / >>Longevity regulating pathway; / >>Longevity regulating pathway - multiple species; / >>Ferroptosis; / >>NOD-like receptor signaling pathway; / >>RIG-I-like receptor signaling pathway; / >>Shigellosis |
| Function | Function:May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.,Function:Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed.,induction:By apoptotic stimuli.,PTM:Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane.,similarity:Belongs to the ATG5 family.,subcellular location:Colocalizes with nonmuscle actin.,tissue specificity:Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells., |
Please login to post questions
Related products
