Name: U-Blot® Aurora B Rabbit mAb
SKU: WR4134
Price: 268 USD
Availability: InStock

U-Blot® Aurora B Rabbit mAb

Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4°C, over night with a 1:2500 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody(Cat:WS0446) was used to detect the antibody.

WR4134 Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4°C, over night with a 1:2500 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody(Cat:WS0446) was used to detect the antibody.

Western blot analysis of lysates from K562 cells, using Aurora B Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.

WR4134 Western blot analysis of lysates from K562 cells, using Aurora B Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.

Price range: $268.00 through $328.00

Size

100μl

50μl

Clear

SKU: WR4134-50

Enter the quantity you would like, or drag the panel to find the quantity and price that best for you

Bulk Quote Authorize OEM & ODM

Product details

Background:This gene encodes a member of the aurora kinase subfamily of serine/threonine kinases. The genes encoding the other two members of this subfamily are located on chromosomes 19 and 20. These kinases participate in the regulation of alignment and segregation of chromosomes during mitosis and meiosis through association with microtubules. A pseudogene of this gene is located on chromosome 8. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2015],

Specifications

Size	50μL/100μL
Target	AurB
Reactivity	Human
Applications	WB, IHC, IF, IP, ELISA
MW(Calculated)	39kD
MW(Observed)	39kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:500-1:1000; WB 1:1000-1:5000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM0246R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	AURKB
Protein Name	Aurora kinase B,Aurora B
Other Name	AURKB; / AIK2; / AIM1; / AIRK2; / ARK2; / STK1; / STK12; / STK5; / Aurora kinase B; / Aurora 1; / Aurora- and IPL1-like midbody-associated protein 1; / AIM-1; / Aurora/IPL1-related kinase 2; / ARK-2; / Aurora-related kinase 2; / STK-1; / Serine/threonine-protein kinase 12
Species	Human
Gene ID	9212
Uniprot	Q96GD4,
Species.1	Mouse
Gene ID.1	20877
Uniprot.1	O70126,
Species.2	Rat
Gene ID.2	114592
Uniprot.2	O55099
Cellular Localization	Nucleus
Tissue Expression	High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.; [Isoform 3]: Not expressed in normal liver, high expression in metastatic liver.
Signaling_pathway	Cellular Processes >> Cell growth and death >> Cell cycle
Research Areas
Function	Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,Disease:Disruptive regulation of expression is a possibile mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis.,Function:May be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Phosphorylates 'Ser-10' and 'Ser-28' of histone H3 during mitosis.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.,similarity:Contains 1 protein kinase domain.,subcellular location:Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body.,subunit:Interacts with TACC1. Associates with RACGAP1 during M phase. Component of the CPC at least composed of BIRC5/survivin CDCA8/borealin, INCENP and AURKB/Aurora-B. Interacts with CDCA1 and NDC80. Interacts with EVI5.,tissue specificity:High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.,

Please login to post questions

U-Blot® Aurora B Rabbit mAb

Product details

Specifications

About Us

Support

Order

Menu

U-Blot® Aurora B Rabbit mAb

Product details

Specifications

Related products

U-Blot® CDK6 Rabbit mAb

U-Blot® GSK3 β (Phospho Ser9) Rabbit mAb

U-Blot™ Phosphate Buffered Saline with Tween 20 (PBS-T), pH 7.4

U-Blot® CDC37 (Phospho Ser13) Rabbit mAb

U-Blot® Ezrin Rabbit mAb

U-Blot™ Universal Antibody Dilution Buffer

Customer Reviews

Menu