Name: U-Blot® BACE Rabbit mAb
SKU: WR4436
Price: 268 USD
Availability: InStock

U-Blot® BACE Rabbit mAb

Hela whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with primary antibody(1:1000). The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.

WR4436 Hela whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with primary antibody(1:1000). The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.

Price range: $268.00 through $328.00

Size

100μl

50μl

Clear

SKU: WR4436-50

Enter the quantity you would like, or drag the panel to find the quantity and price that best for you

Bulk Quote Authorize OEM & ODM

Product details

Background:beta-secretase 1(BACE1) Homo sapiens This gene encodes a member of the peptidase A1 family of aspartic proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protease. This transmembrane protease catalyzes the first step in the formation of amyloid beta peptide from amyloid precursor protein. Amyloid beta peptides are the main constituent of amyloid beta plaques, which accumulate in the brains of human Alzheimer's disease patients. [provided by RefSeq, Nov 2015],

Specifications

Size	50μL/100μL
Target	BACE
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, IP, ELISA
MW(Calculated)	56kD
MW(Observed)	70kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:100-1:400; WB 1:1000-1:5000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM0655R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	BACE1
Protein Name	Beta-secretase 1
Other Name	BACE1; / BACE; / KIAA1149; / Beta-secretase 1; / Aspartyl protease 2; / ASP2; / Asp 2; / Beta-site amyloid precursor protein cleaving enzyme 1; / Beta-site APP cleaving enzyme 1; / Memapsin-2; / Membrane-associated aspartic protease 2
Species	Human
Gene ID	23621
Uniprot	P56817,
Species.1	Mouse
Gene ID.1	23821
Uniprot.1	P56818,
Species.2	Rat
Gene ID.2	29392
Uniprot.2	P56819
Cellular Localization	Membrane, Cytoplasm
Tissue Expression	Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata.
Signaling_pathway	Human Diseases >> Neurodegenerative disease >> Alzheimer disease
Research Areas	>>Alzheimer disease
Function	Catalytic activity:Broad endopeptidase specificity. Cleaves Glu-Val-Asn-Leu-\|-Asp-Ala-Glu-Phe in the Swedish variant of Alzheimer's amyloid precursor protein.,enzyme regulation:Inhibited by RTN3 and RTN4.,Function:Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase.,similarity:Belongs to the peptidase A1 family.,subunit:Monomer. Interacts with GGA1, GGA2 and GGA3. Interacts with RTN3 and RTN4.,tissue specificity:Brain.,