Bag-3 Polyclonal Antibody
Ucallm_WP15555-50
Price range: $268.00 through $328.00
Product details
Background:
BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | Bag-3 |
| Reactivity | Human, Mouse |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 80kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC: 1:100-1:300; ELISA 1:10000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human BAG3. AA range:311-360 |
| Specificity | Bag-3 Polyclonal Antibody detects endogenous levels of Bag-3 protein. |
| Gene Name | BAG3 BIS |
| Protein Name | BAG family molecular chaperone regulator 3 |
| Other Name | BAG3; / BIS; / BAG family molecular chaperone regulator 3; / BAG-3; / Bcl-2-associated athanogene 3; / Bcl-2-binding protein Bis; / Docking protein CAIR-1 |
| Species | Human |
| Gene ID | 9531 |
| Uniprot | O95817, |
| Species.1 | Mouse |
| Gene ID.1 | 29810 |
| Uniprot.1 | Q9JLV1 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus . Cytoplasm . Colocalizes with HSF1 to the nucleus upon heat stress (PubMed:26159920). . |
| Tissue Expression | Brain,Epithelium,Liver,Lung,Placenta,T-cell,Testis,Tongue, |
| Signaling_pathway | |
| Research Areas | |
| Function | Function:Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Has anti-apoptotic activity.,similarity:Contains 1 BAG domain.,similarity:Contains 2 WW domains.,subunit:Binds to the ATPase domain of HSP70/HSC chaperones. Binds to Bcl-2 and PLC-gamma., |
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