Beclin-1 Monoclonal Antibody
Ucallm_WM0049-50
Price range: $268.00 through $328.00
Product details
Background:
beclin 1(BECN1) Homo sapiens This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015],
Specifications
| Size | 50μL/100μL |
| Target | Beclin 1 |
| Reactivity | Human |
| Applications | WB, IHC, IF, FC, ELISA |
| MW(Calculated) | 52kD |
| MW(Observed) | |
| Host Species | Mouse |
| Isotype | |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:200-1:1000; Flow Cyt 1:200-1:400; ELISA 1:10000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | Affinity purification |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | 2D11 |
| Immunogen | Purified recombinant fragment of human Beclin-1 expressed in E. Coli. |
| Specificity | Beclin-1 Monoclonal Antibody detects endogenous levels of Beclin-1 protein. |
| Gene Name | BECN1 |
| Protein Name | Beclin-1 |
| Other Name | BECN1; / GT197; / Beclin-1; / Coiled-coil myosin-like BCL2-interacting protein; / Protein GT197 |
| Species | Human |
| Gene ID | 8678 |
| Uniprot | Q14457, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | O88597 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm . Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein . Endosome membrane; Peripheral membrane protein . Endoplasmic reticulum membrane; Peripheral membrane protein . Mitochondrion membrane; Peripheral membrane protein . Endosome . Cytoplasmic vesicle, autophagosome . Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells (By similarity). .; [Beclin-1-C 35 kDa]: Mitochondrion . Nucleus . Cytoplasm .; [Beclin-1-C 37 kDa]: Mitochondrion . |
| Tissue Expression | Ubiquitous. |
| Signaling_pathway | Cellular Processes >> Transport and catabolism >> Autophagy - animal;Cellular Processes >> Transport and catabolism >> Mitophagy - animal;Cellular Processes >> Cell growth and death >> Apoptosis - multiple species;Human Diseases >> Neurodegenerative disease >> Alzheimer disease;Human Diseases >> Neurodegenerative disease >> Amyotrophic lateral sclerosis;Human Diseases >> Neurodegenerative disease >> Huntington disease;Human Diseases >> Neurodegenerative disease >> Spinocerebellar ataxia;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases;Environmental Information Processing >> Signal transduction >> Apelin signaling pathway |
| Research Areas | >>Autophagy - other; / >>Mitophagy - animal; / >>Autophagy - animal; / >>Apoptosis - multiple species; / >>Apelin signaling pathway; / >>Alzheimer disease; / >>Amyotrophic lateral sclerosis; / >>Huntington disease; / >>Spinocerebellar ataxia; / >>Pathways of neurodegeneration - multiple diseases; / >>Shigellosis; / >>Kaposi sarcoma-associated herpesvirus infection |
| Function | Function:Plays a central role in autophagy (By similarity). May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.,similarity:Belongs to the beclin family.,subcellular location:Expressed in dendrites and cell bodies of cerebellar Purkinje cells.,subunit:Interacts with GOPC and GRID2. Interacts with AMBRA1. Probably forms a complex with AMBRA1 and PIK3C3 (By similarity). Interacts with BCL2 and BCL2L1.,tissue specificity:Ubiquitous., |
Please login to post questions
Related products
