U-Blot® Beclin 1 Rabbit mAb
WR4306 Western blot analysis of lysates from Hela cells, using Beclin 1 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002,) was used to detect the antibody.
Price range: $268.00 through $328.00
Product details
Background:beclin 1(BECN1) Homo sapiens This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015],
Specifications
| Size | 50μL/100μL |
| Target | Beclin 1 |
| Reactivity | Human, Mouse, Rat, Chicken |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 52kD |
| MW(Observed) | 60kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0506R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | BECN1 GT197 |
| Protein Name | Beclin-1 |
| Other Name | Beclin-1 (Coiled-coil myosin-like BCL2-interacting protein) (Protein GT197) |
| Species | Human |
| Gene ID | 8678 |
| Uniprot | Q14457, |
| Species.1 | Mouse |
| Gene ID.1 | 56208 |
| Uniprot.1 | O88597, |
| Species.2 | Rat |
| Gene ID.2 | 114558 |
| Uniprot.2 | Q91XJ1 |
| Cellular Localization | Cytoplasm |
| Tissue Expression | Ubiquitous. |
| Signaling_pathway | Cellular Processes >> Transport and catabolism >> Autophagy - animal;Cellular Processes >> Transport and catabolism >> Mitophagy - animal;Cellular Processes >> Cell growth and death >> Apoptosis - multiple species;Human Diseases >> Neurodegenerative disease >> Alzheimer disease;Human Diseases >> Neurodegenerative disease >> Amyotrophic lateral sclerosis;Human Diseases >> Neurodegenerative disease >> Huntington disease;Human Diseases >> Neurodegenerative disease >> Spinocerebellar ataxia;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases;Environmental Information Processing >> Signal transduction >> Apelin signaling pathway |
| Research Areas | >>Autophagy - other; / >>Mitophagy - animal; / >>Autophagy - animal; / >>Apoptosis - multiple species; / >>Apelin signaling pathway; / >>Alzheimer disease; / >>Amyotrophic lateral sclerosis; / >>Huntington disease; / >>Spinocerebellar ataxia; / >>Pathways of neurodegeneration - multiple diseases; / >>Shigellosis; / >>Kaposi sarcoma-associated herpesvirus infection |
| Function | Function:Plays a central role in autophagy (By similarity). May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.,similarity:Belongs to the beclin family.,subcellular location:Expressed in dendrites and cell bodies of cerebellar Purkinje cells.,subunit:Interacts with GOPC and GRID2. Interacts with AMBRA1. Probably forms a complex with AMBRA1 and PIK3C3 (By similarity). Interacts with BCL2 and BCL2L1.,tissue specificity:Ubiquitous., |
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