Price range: $268.00 through $328.00
Background:
betaine–homocysteine S-methyltransferase(BHMT) Homo sapiens This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed. [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | BHMT |
| Reactivity | Human |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 45kD |
| MW(Observed) | |
| Host Species | Mouse |
| Isotype | |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:200-1:1000; ELISA 1:10000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | Affinity purification |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | 8H8 |
| Immunogen | Purified recombinant fragment of BHMT expressed in E. Coli. |
| Specificity | BHMT Monoclonal Antibody detects endogenous levels of BHMT protein. |
| Gene Name | BHMT |
| Protein Name | Betaine--homocysteine S-methyltransferase 1 |
| Other Name | BHMT; / Betaine--homocysteine S-methyltransferase 1 |
| Species | Human |
| Gene ID | 635 |
| Uniprot | Q93088, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | O35490 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm, cytosol . Nucleus . Predominantly localized in the cytoplasm with a small fraction detected in the nucleus. Translocates into the nucleus upon oxidative stress. . |
| Tissue Expression | Found exclusively in liver and kidney. |
| Signaling_pathway | |
| Research Areas | >>Glycine, serine and threonine metabolism; / >>Cysteine and methionine metabolism; / >>Metabolic pathways |
| Function | Catalytic activity:Trimethylammonioacetate + L-homocysteine = dimethylglycine + L-methionine.,cofactor:Binds 1 zinc ion per subunit.,Function:Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.,pathway:Amine and polyamine degradation; betaine degradation; sarcosine from betaine: step 1/2.,pathway:Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (BhmT route): step 1/1.,similarity:Contains 1 Hcy-binding domain.,subunit:Homotetramer.,tissue specificity:Found exclusively in liver and kidney., |
