Name: U-Blot® BMAL1 Rabbit mAb
SKU: WR4476
Price: 268 USD
Availability: InStock

Product details

Background:The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This heterodimer binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Defects in this gene have been linked to infertility, problems with gluconeogenesis and lipogenesis, and altered sleep patterns. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014],

Specifications

Size	50μL/100μL
Target	BMAL1
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, IP, ELISA
MW(Calculated)	69kD
MW(Observed)	78kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM0696R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	ARNTL
Protein Name	Aryl hydrocarbon receptor nuclear translocator-like protein 1
Other Name	ARNTL; / BHLHE5; / BMAL1; / MOP3; / PASD3; / Aryl hydrocarbon receptor nuclear translocator-like protein 1; / Basic-helix-loop-helix-PAS protein MOP3; / Brain and muscle ARNT-like 1Class E basic helix-loop-helix protein 5; / bHLHe5; / Member of PAS protein 3; / PAS domain-containing protein 3; / bHLH-PAS protein JAP3
Species	Human
Gene ID	406
Uniprot	O00327,
Species.1	Mouse
Gene ID.1	11865
Uniprot.1	Q9WTL8,
Species.2	Rat
Gene ID.2	29657
Uniprot.2	Q9EPW1
Cellular Localization	Nucleus
Tissue Expression	Hair follicles (at protein level). Highly expressed in the adult brain, skeletal muscle and heart.
Signaling_pathway	Organismal Systems >> Nervous system >> Dopaminergic synapse;Organismal Systems >> Environmental adaptation >> Circadian rhythm
Research Areas	>>Circadian rhythm; / >>Dopaminergic synapse
Function	Alternative products:Additional isoforms seem to exist,Function:ARNTL-CLOCK heterodimers activate E-box element (3'-CACGTG-5') transcription of a number of proteins of the circadian clock. This transcription is inhibited in a feedback loop by PER, and also by CRY proteins.,miscellaneous:CLOCK-ARNTL double mutations within the PAS domains result in syngernistic desensitization to high levels of CRY on repression of CLOCK-ARNTL transcriptional activity of PER1 and, disrupt circadian rhythmicity.,PTM:Acetylated on Lys-538 upon dimerization with CLOCK. Acetylation facilitates CRY1-mediated repression.,PTM:Phosphorylated upon dimerization with CLOCK.,PTM:Sumoylated on Lys-259 upon dimerization with CLOCK.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,similarity:Contains 1 PAC (PAS-associated C-terminal) domain.,similarity:Contains 2 PAS (PER-ARNT-SIM) domains.,subunit:Component of the circadian clock oscillator which includes the CRY proteins, CLOCK or NPAS2, ARNTL or ARNTL2, CSNK1D and/or CSNK1E, TIMELESS and the PER proteins. Efficient DNA binding requires dimerization with another bHLH protein. Heterodimerization with CLOCK is required for E-box-dependent transactivation, for CLOCK nuclear translocation and degradation, and, for phosphorylation of both CLOCK and ARNTL. Interaction with PER and CRY proteins requires translocation to the nucleus. Interaction of the CLOCK-ARNTL heterodimer with PER or CRY inhibits transcription activation. Interacts with HSP90; with AHR in vitro, but not in vivo.,tissue specificity:Highly expressed in the adult brain, skeletal muscle and heart.,