Price range: $268.00 through $328.00
Background:
The protein encoded by this gene was identified by its ability to bind to the nuclear localization signal of BRCA1 and other proteins. It is a cytoplasmic protein which may regulate nuclear targeting by retaining proteins with a nuclear localization signal in the cytoplasm. [provided by RefSeq, Jul 2008],
| Target | BRAP |
| Reactivity | Human |
| Applications | WB |
| MW(Calculated) | |
| MW(Observed) | 67kD |
| Host Species | Mouse |
| Isotype | |
| Conjugate/Modification | Unmodified |
| Modification | |
| Recommended Dilution Ratio | WB 1:1000 |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Source | |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen. |
| Purity | |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | 9H8 |
| Immunogen | Purified recombinant human BRAP protein fragments expressed in E.coli. |
| Sequence | |
| Specificity | This antibody detects endogenous levels of BRAP and does not cross-react with related proteins. |
| Gene Name | brap |
| Protein Name | |
| Other Name | 3010002G07Rik; / BRAP2; / BRCA1 associated protein; / Galectin 2 binding protein; / IMP; / Impedes mitogenic signal propagation; / Renal carcinoma antigen NY REN 63; / RING finger protein 52; / RNF52; / zgc:92894. |
| Species | Human |
| Gene ID-1 | 8315 |
| Uniprot | Q7Z569, |
| Species.1 | Mouse |
| Gene ID-2 | |
| Uniprot.1 | Q99MP8 |
| Species.2 | |
| Gene ID-3 | |
| Uniprot.2 | |
| Organism-4 | |
| Gene ID-4 | |
| SwissProt-4 | |
| Background | The protein encoded by this gene was identified by its ability to bind to the nuclear localization signal of BRCA1 and other proteins. It is a cytoplasmic protein which may regulate nuclear targeting by retaining proteins with a nuclear localization signal in the cytoplasm. [provided by RefSeq, Jul 2008], |
| Cellular Localization | Cytoplasm . |
| Tissue Expression | Expressed in breast epithelial cell lines. |
| Signaling_pathway | Environmental Information Processing >> Signal transduction >> Ras signaling pathway |
| Research Areas | >>Ras signaling pathway |
| Function | Function:Negatively regulates MAP kinase activation by limiting the formation of Raf/MEK complexes probably by inactivation of the KSR1 scaffold protein. Also acts as a Ras responsive E3 ubiquitin ligase that, on activation of Ras, is modified by auto-polyubiquitination resulting in the release of inhibition of Raf/MEK complex formation. May also act as a cytoplasmic retention protein with a role in regulating nuclear transport.,pathway:Protein modification; protein ubiquitination.,similarity:Contains 1 RING-type zinc finger.,similarity:Contains 1 UBP-type zinc finger.,subunit:Interacts with the nuclear localization signal of BRCA1 and with the N-terminal of KSR1. The C-terminal portion of BCRA1 interacts with DDB1.,tissue specificity:Expressed in breast epithelial cell lines., |
| RRID |
