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U-Blot® BRAT1 Rabbit mAb
WR4716 Western Blot analysis of HeLa whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-BRAT1 rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.
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Product details
Background:The protein encoded by this ubiquitously expressed gene interacts with the tumor suppressing BRCA1 (breast cancer 1) protein and and the ATM (ataxia telangiectasia mutated) protein. ATM is thought to be a master controller of cell cycle checkpoint signalling pathways that are required for cellular responses to DNA damage such as double-strand breaks that are induced by ionizing radiation and complexes with BRCA1 in the multi-protein complex BASC (BRAC1-associated genome surveillance complex). The protein encoded by this gene is thought to play a role in the DNA damage pathway regulated by BRCA1 and ATM. [provided by RefSeq, Mar 2012]
Specifications
| Size | 50μL/100μL |
| Target | BRAT1 |
| Reactivity | Human |
| Applications | WB, IF, ICC, FC, IP |
| MW(Calculated) | |
| MW(Observed) | 90kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:1000-5000; ICC/IF 1:100-300; FC 1:100-300; IP 1:50-100 |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0803R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | BRAT1; BAAT1; C7orf27 |
| Protein Name | BRCA1-associated ATM activator 1; BRCA1-associated protein required for ATM activation protein 1 |
| Other Name | BRAT1; / BAAT1; / C7orf27; / BRCA1-associated ATM activator 1; / BRCA1-associated protein required for ATM activation protein 1 |
| Species | Human |
| Gene ID | 221927 |
| Uniprot | Q6PJG6 |
| Species.1 | |
| Gene ID.1 | |
| Uniprot.1 | |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus. Cytoplasm. Note=Present at double strand breaks (DSBs)following ionizing radiation treatment. The ubiquitinated form localizes in the nucleus in a NDFIP1-dependent manner.. |
| Tissue Expression | Ubiquitously expressed. {ECO:0000269|PubMed:16452482}. |
| Signaling_pathway | |
| Research Areas | |
| Function | Involved in DNA damage response; activates kinases ATM, SMC1A and PRKDC by modulating their phosphorylation status following ionizing radiation (IR) stress (PubMed:16452482, PubMed:22977523). Plays a role in regulating mitochondrial function and cell proliferation (PubMed:25070371). Required for protein stability of MTOR and MTOR-related proteins, and cell cycle progress by growth factors (PubMed:25657994). {ECO:0000269|PubMed:16452482, ECO:0000269|PubMed:22977523, ECO:0000269|PubMed:25070371, ECO:0000269|PubMed:25657994}. |
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