Name: U-Blot® c-Fos Rabbit mAb
SKU: WR4263
Price: 268 USD
Availability: InStock

Product details

Background:The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq, Jul 2008],

Specifications

Size	50μL/100μL
Target	c-Fos
Reactivity	Human, Mouse, Rat
Applications	WB, IF, IP, ELISA
MW(Calculated)	41kD
MW(Observed)	55kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200;
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM0451R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	FOS
Protein Name	Proto-oncogene c-Fos
Other Name	FOS; / G0S7; / Proto-oncogene c-Fos; / Cellular oncogene fos; / G0/G1 switch regulatory protein 7
Species	Human
Gene ID	2353
Uniprot	P01100,
Species.1	Mouse
Gene ID.1	14281
Uniprot.1	P01101,
Species.2	Rat
Gene ID.2	140675
Uniprot.2	P12841
Cellular Localization	Nucleus
Tissue Expression	Lung adenocarcinoma,Pancreas,Tongue,
Signaling_pathway	Cellular Processes >> Cell growth and death >> Apoptosis;Organismal Systems >> Immune system >> Toll-like receptor signaling pathway;Organismal Systems >> Immune system >> T cell receptor signaling pathway;Organismal Systems >> Immune system >> Th1 and Th2 cell differentiation;Organismal Systems >> Immune system >> Th17 cell differentiation;Organismal Systems >> Immune system >> IL-17 signaling pathway;Organismal Systems >> Immune system >> B cell receptor signaling pathway;Organismal Systems >> Endocrine system >> Estrogen signaling pathway;Organismal Systems >> Endocrine system >> Prolactin signaling pathway;Organismal Systems >> Endocrine system >> Oxytocin signaling pathway;Organismal Systems >> Endocrine system >> Relaxin signaling pathway;Organismal Systems >> Endocrine system >> Growth hormone synthesis, secretion and action;Organismal Systems >> Nervous system >> Cholinergic synapse;Organismal Systems >> Nervous system >> Dopaminergic synapse;Organismal Systems >> Development and regeneration >> Osteoclast differentiation;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: overview >> PD-L1 expression and PD-1 checkpoint pathway in cancer;Human Diseases >> Cancer: specific types >> Colorectal cancer;Human Diseases >> Cancer: specific types >> Breast cancer;Human Diseases >> Immune disease >> Rheumatoid arthritis;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway;Environmental Information Processing >> Signal transduction >> TNF signaling pathway;Environmental Information Processing >> Signal transduction >> cAMP signaling pathway
Research Areas	>>Endocrine resistance; / >>MAPK signaling pathway; / >>cAMP signaling pathway; / >>Apoptosis; / >>Osteoclast differentiation; / >>Toll-like receptor signaling pathway; / >>IL-17 signaling pathway; / >>Th1 and Th2 cell differentiation; / >>Th17 cell differentiation; / >>T cell receptor signaling pathway; / >>B cell receptor signaling pathway; / >>TNF signaling pathway; / >>Circadian entrainment; / >>Cholinergic synapse; / >>Dopaminergic synapse; / >>Estrogen signaling pathway; / >>Prolactin signaling pathway; / >>Oxytocin signaling pathway; / >>Relaxin signaling pathway; / >>Parathyroid hormone synthesis, secretion and action; / >>Non-alcoholic fatty liver disease; / >>Growth hormone synthesis, secretion and action; / >>Amphetamine addiction; / >>Pathogenic Escherichia coli infection; / >>Salmonella infection; / >>Pertussis; / >>Yersinia infection; / >>Leishmaniasis; / >>Chagas disease; / >>Hepatitis B; / >>Measles; / >>Human T-cell leukemia virus 1 infection; / >>Kaposi sarcoma-associated herpesvirus infection; / >>Human immunodeficiency virus 1 infection; / >>Coronavirus disease - COVID-19; / >>Pathways in cancer; / >>Chemical carcinogenesis - receptor activation; / >>Chemical carcinogenesis - reactive oxygen species; / >>Colorectal cancer; / >>Breast cancer; / >>Choline metabolism in cancer; / >>PD-L1 expression and PD-1 checkpoint pathway in cancer; / >>Rheumatoid arthritis; / >>Lipid and atherosclerosis; / >>Fluid shear stress and atherosclerosis
Function	Function:Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, c-fos and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation.,PTM:Constitutively sumoylated by SUMO1, SUMO2 and SUMO3. Desumoylated by SENP2. Sumoylation requires heterodimerization with JUN and is enhanced by mitogen stimulation. Sumoylation inhibits the AP-1 transcriptional activity and is, itself, inhibited by Ras-activated phosphorylation on Thr-232.,PTM:Phosphorylated in the C-terminal upon stimulation by nerve growth factor (NGF) and epidermal growth factor (EGF). Phosphorylated, in vitro, by MAPK and RSK1. Phosphorylation on both Ser-362 and Ser-374 by MAPK1/2 and RSK1/2 leads to protein stabilization with phosphorylation on Ser-374 being the major site for protein stabilization on NGF stimulation. Phosphorylation on Ser-362 and Ser-374 primes further phosphorylations on Thr-325 and Thr-331 through promoting docking of MAPK to the DEF domain. Phosphorylation on Thr-232, induced by HA-RAS, activates the transcriptional activity and antagonizes sumoylation. Phosphorylation on Ser-362 by RSK2 in osteoblasts contributes to osteoblast transformation.,similarity:Belongs to the bZIP family.,similarity:Belongs to the bZIP family. Fos subfamily.,similarity:Contains 1 bZIP domain.,subunit:Heterodimer with JUN. Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA. Interacts with MAFB.,