Name: c-Src Polyclonal Antibody
SKU: WP11820
Price: 268 USD
Availability: InStock

Product details

Background:

This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008],

Specifications

Target	c-Src
Reactivity	Human, Mouse, Rat
Applications	WB, IP, IHC, IF, ELISA
MW(Calculated)
MW(Observed)	60kD
Host Species	Rabbit
Isotype	IgG
Conjugate/Modification	Unmodified
Modification
Recommended Dilution Ratio	WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:40000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration	1 mg/ml
Clonality	Polyclonal
Clone Number
Immunogen	Synthesized peptide derived from c-Src . at AA range: 360-440
Sequence
Specificity	c-Src Polyclonal Antibody detects endogenous levels of c-Src protein.
Gene Name	SRC SRC1
Protein Name	Proto-oncogene tyrosine-protein kinase Src
Other Name	SRC; / SRC1; / Proto-oncogene tyrosine-protein kinase Src; / Proto-oncogene c-Src; / pp60c-src; / p60-Src
Species	Human
Gene ID-1	6714
Uniprot	P12931,
Species.1	Mouse
Gene ID-2	20779
Uniprot.1	P05480,
Species.2	Rat
Gene ID-3
Uniprot.2	Q9WUD9
Organism-4
Gene ID-4
SwissProt-4
Background	This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008],
Cellular Localization	Cell membrane; Lipid-anchor . Mitochondrion inner membrane . Nucleus . Cytoplasm, cytoskeleton . Cytoplasm, perinuclear region . Cell junction, focal adhesion . Localizes to focal adhesion sites following integrin engagement (PubMed:22801373). Localization to focal adhesion sites requires myristoylation and the SH3 domain (PubMed:7525268). Colocalizes with PDLIM4 at the perinuclear region, but not at focal adhesions (PubMed:19307596). .
Tissue Expression	Expressed ubiquitously. Platelets, neurons and osteoclasts express 5-fold to 200-fold higher levels than most other tissues.; [Isoform 1]: Expressed in spleen and liver.; [Isoform 2]: Expressed in brain.; [Isoform 3]: Expressed in brain.
Signaling_pathway	Cellular Processes >> Transport and catabolism >> Mitophagy - animal;Cellular Processes >> Cellular community - eukaryotes >> Focal adhesion;Cellular Processes >> Cellular community - eukaryotes >> Adherens junction;Cellular Processes >> Cellular community - eukaryotes >> Tight junction;Cellular Processes >> Cellular community - eukaryotes >> Gap junction;Cellular Processes >> Cell motility >> Regulation of actin cytoskeleton;Organismal Systems >> Immune system >> Platelet activation;Organismal Systems >> Immune system >> Neutrophil extracellular trap formation;Organismal Systems >> Immune system >> Chemokine signaling pathway;Organismal Systems >> Endocrine system >> GnRH signaling pathway;Organismal Systems >> Endocrine system >> Estrogen signaling pathway;Organismal Systems >> Endocrine system >> Prolactin signaling pathway;Organismal Systems >> Endocrine system >> Oxytocin signaling pathway;Organismal Systems >> Endocrine system >> Relaxin signaling pathway;Organismal Systems >> Endocrine system >> Thyroid hormone signaling pathway;Organismal Systems >> Sensory system >> Inflammatory mediator regulation of TRP channels;Human Diseases >> Cancer: specific types >> Bladder cancer;Environmental Information Processing >> Signal transduction >> ErbB signaling pathway;Environmental Information Processing >> Signal transduction >> Rap1 signaling pathway;Environmental Information Processing >> Signal transduction >> VEGF signaling pathway
Research Areas	>>EGFR tyrosine kinase inhibitor resistance; / >>Endocrine resistance; / >>ErbB signaling pathway; / >>Rap1 signaling pathway; / >>Chemokine signaling pathway; / >>Mitophagy - animal; / >>Endocytosis; / >>Axon guidance; / >>VEGF signaling pathway; / >>Focal adhesion; / >>Adherens junction; / >>Tight junction; / >>Gap junction; / >>Platelet activation; / >>Neutrophil extracellular trap formation; / >>C-type lectin receptor signaling pathway; / >>GABAergic synapse; / >>Inflammatory mediator regulation of TRP channels; / >>Regulation of actin cytoskeleton; / >>GnRH signaling pathway; / >>Estrogen signaling pathway; / >>Prolactin signaling pathway; / >>Thyroid hormone signaling pathway; / >>Oxytocin signaling pathway; / >>Relaxin signaling pathway; / >>Bacterial invasion of epithelial cells; / >>Epithelial cell signaling in Helicobacter pylori infection; / >>Pathogenic Escherichia coli infection; / >>Shigellosis; / >>Yersinia infection; / >>Tuberculosis; / >>Hepatitis B; / >>Human cytomegalovirus infection; / >>Kaposi sarcoma-associated herpesvirus infection; / >>Herpes simplex virus 1 infection; / >>Viral carcinogenesis; / >>Proteoglycans in cancer; / >>Chemical carcinogenesis - receptor activation; / >>Chemical carcinogenesis - reactive oxygen species; / >>Bladder cancer; / >>Lipid and atherosclerosis; / >>Fluid shear stress and atherosclerosis
Function	Catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,PTM:Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. The phosphorylated tail interacts with the SH2 domain thereby repressing kinase activity.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SH2 domain.,similarity:Contains 1 SH3 domain.,subunit:Interacts with DDEF1/ASAP1; via the SH3 domain. Interacts with CCPG1 (By similarity). Interacts with CDCP1, PELP1, TGFB1I1 and TOM1L2. Interacts with the cytoplasmic domain of MUC1, phosphorylates it and increases binding of MUC1 with beta-catenin. Interacts with RALGPS1; via the SH3 domain. Interacts with HEV ORF3 protein; via the SH3 domain.,
RRID	AB_2927476

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c-Src Polyclonal Antibody

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c-Src Polyclonal Antibody

Product details

Specifications

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