U-Blot® CACNA1F Rabbit mAb
WR4558 Western Blot analysis of HL-60 whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CACNA1F rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.
Price range: $268.00 through $328.00
Product details
Background:calcium voltage-gated channel subunit alpha1 F(CACNA1F) Homo sapiens This gene encodes a multipass transmembrane protein that functions as an alpha-1 subunit of the voltage-dependent calcium channel, which mediates the influx of calcium ions into the cell. The encoded protein forms a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Mutations in this gene can cause X-linked eye disorders, including congenital stationary night blindness type 2A, cone-rod dystropy, and Aland Island eye disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Aug 2013],
Specifications
| Size | 50μL/100μL |
| Target | CACNA1F |
| Reactivity | Human |
| Applications | WB, FC |
| MW(Calculated) | |
| MW(Observed) | 217kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:1000-5000; FC 1:100-300 |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0769R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | CACNA1F; CACNAF1 |
| Protein Name | Voltage-dependent L-type calcium channel subunit alpha-1F; Voltage-gated calcium channel subunit alpha Cav1.4 |
| Other Name | CACNA1F; / CACNAF1; / Voltage-dependent L-type calcium channel subunit alpha-1F; / Voltage-gated calcium channel subunit alpha Cav1.4 |
| Species | Human |
| Gene ID | 778 |
| Uniprot | O60840, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q9JIS7 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Membrane; Multi-pass membrane protein. |
| Tissue Expression | Expression in skeletal muscle and retina (PubMed:10873387). Isoform 4 is expressed in retina (PubMed:27226626). {ECO:0000269|PubMed:10873387, ECO:0000269|PubMed:27226626}. |
| Signaling_pathway | Organismal Systems >> Endocrine system >> Insulin secretion;Organismal Systems >> Endocrine system >> GnRH signaling pathway;Organismal Systems >> Endocrine system >> Oxytocin signaling pathway;Organismal Systems >> Endocrine system >> Growth hormone synthesis, secretion and action;Organismal Systems >> Endocrine system >> Aldosterone synthesis and secretion;Organismal Systems >> Circulatory system >> Cardiac muscle contraction;Organismal Systems >> Circulatory system >> Adrenergic signaling in cardiomyocytes;Organismal Systems >> Circulatory system >> Vascular smooth muscle contraction;Organismal Systems >> Nervous system >> Cholinergic synapse;Human Diseases >> Neurodegenerative disease >> Alzheimer disease;Human Diseases >> Neurodegenerative disease >> Prion disease;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway;Environmental Information Processing >> Signal transduction >> Calcium signaling pathway;Environmental Information Processing >> Signal transduction >> cAMP signaling pathway;Environmental Information Processing >> Signal transduction >> cGMP-PKG signaling pathway |
| Research Areas | >>MAPK signaling pathway; / >>Calcium signaling pathway; / >>cGMP-PKG signaling pathway; / >>cAMP signaling pathway; / >>Cardiac muscle contraction; / >>Adrenergic signaling in cardiomyocytes; / >>Vascular smooth muscle contraction; / >>Retrograde endocannabinoid signaling; / >>Cholinergic synapse; / >>Serotonergic synapse; / >>GABAergic synapse; / >>Insulin secretion; / >>GnRH signaling pathway; / >>Oxytocin signaling pathway; / >>Renin secretion; / >>Aldosterone synthesis and secretion; / >>Cortisol synthesis and secretion; / >>GnRH secretion; / >>Cushing syndrome; / >>Growth hormone synthesis, secretion and action; / >>Alzheimer disease; / >>Prion disease; / >>Pathways of neurodegeneration - multiple diseases; / >>Chemical carcinogenesis - receptor activation; / >>Hypertrophic cardiomyopathy; / >>Arrhythmogenic right ventricular cardiomyopathy; / >>Dilated cardiomyopathy |
| Function | [Isoform 1]: Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1F gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, and by benzothiazepines. Activates at more negative voltages and does not undergo calcium-dependent inactivation (CDI), due to incoming calcium ions, during depolarization. {ECO:0000269|PubMed:15897456, ECO:0000269|PubMed:27226626}.; [Isoform 4]: Voltage-dependent L-type calcium channel activates at more hyperpolarized voltages and exhibits a robust calcium-dependent inactivation (CDI), due to incoming calcium ions, during depolarizations. {ECO:0000269|PubMed:27226626}.; [Isoform 5]: Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. {ECO:0000269|PubMed:27226626}.; [Isoform 6]: Voltage-dependent L-type calcium channel activates at more hyperpolarized voltages and exhibits a robust calcium-dependent inactivation (CDI), due to incoming calcium ions, during depolarizations. {ECO:0000269|PubMed:27226626}. |
Please login to post questions
Related products
