CAF-1 p60 Polyclonal Antibody
Ucallm_WP11378-50
Price range: $268.00 through $328.00
Product details
Background:
Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | CAF-1 p60 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 61kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:20000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CAF1B. AA range:71-120 |
| Specificity | CAF-1 p60 Polyclonal Antibody detects endogenous levels of CAF-1 p60 protein. |
| Gene Name | CHAF1B |
| Protein Name | Chromatin assembly factor 1 subunit B |
| Other Name | CHAF1B; / CAF1A; / CAF1P60; / MPHOSPH7; / MPP7; / Chromatin assembly factor 1 subunit B; / CAF-1 subunit B; / Chromatin assembly factor I p60 subunit; / CAF-I 60 kDa subunit; / CAF-I p60; / M-phase phosphoprotein 7 |
| Species | Human |
| Gene ID | 8208 |
| Uniprot | Q13112, |
| Species.1 | Mouse |
| Gene ID.1 | 110749 |
| Uniprot.1 | Q9D0N7 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus . Cytoplasm . DNA replication foci. Cytoplasmic in M phase. |
| Tissue Expression | Epithelium,Lymphoblast,Uterus, |
| Signaling_pathway | |
| Research Areas | |
| Function | developmental stage:Active complex is found in G1, S and G2 phases.,Function:Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. The CCR4-NOT complex functions as general transcription regulation complex.,PTM:Differentially phosphorylated during cell cycle. During mitosis the p60 subunit of inactive CAF-1 is hyperphosphorylated and displaced into the cytosol. Progressivly dephosphorylated from G1 to S and G2 phase. Phosphorylated p60 is recruited to chromatin undergoing DNA repair after UV irradiation in G1, S or G2 phases.,similarity:Belongs to the WD repeat HIR1 family.,similarity:Contains 7 WD repeats.,subcellular location:DNA replication foci. Cytoplasmic in M phase.,subunit:Subunit of the CAF-1 complex that contains RBBP4, CHAF1B and CHAF1A. CHAF1A binds directly to CHAF1B. Only minor amounts of RBBP4 are complexed with CHAF1A and CHAF1B in G1 phase. In G2 and S phase also monomeric CHAF1B is detected. Subunit of the CCR4-NOT core complex that contains CHAF1A, CHAF1B, CNOT1, CNOT2, CNOT3, CNOT4, CNOT6 and CNOT8., |
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