Price range: $268.00 through $328.00
Background:
Calponin-1 is a calcium regulatory protein that binds tropomyosin and F-actin. It is a specific protein of smooth muscle cells and has the function of inhibiting the contraction of smooth muscle cells. It is expressed in smooth muscle and myoepithelial cells, as well as myofibroblasts. It is mainly used to study the distribution of myoepithelial cells in smooth muscle tumors and breast tissues.
| Size | 50μL/100μL |
| Target | Calponin-1 |
| Reactivity | Human |
| Applications | IHC, WB, IF, ELISA |
| MW(Calculated) | 33kD |
| MW(Observed) | 34kD |
| Host Species | Mouse |
| Isotype | IgG2a,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1000; WB 1:500-2000; IF 1:100-500; ELISA 1:1000-5000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein G affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RMM133 |
| Immunogen | Synthesized peptide derived from human Calponin-1 AA range: 100-200 |
| Specificity | The antibody can specifically recognize human and mouse calponin-1 protein. In western blotting of Hela, LnCap and mouse muscle lysates, the antibody can label a 34 kDa band corresponding to calponin-1. |
| Gene Name | CNN1 |
| Protein Name | Calponin-1 |
| Other Name | Basic calponin; / Calponin 1; / Calponin 1 basic smooth muscle; / Calponin H1; / Calponin H1 smooth muscle; / Calponin-1; / Calponin1; / Calponins basic; / CNN 1; / Cnn1; / CNN1_HUMAN; / Epididymis secretory protein Li 14; / HEL S 14; / Sm Calp; / SMCC; / smooth muscle |
| Species | Human |
| Gene ID | 1264 |
| Uniprot | P51911 |
| Species.1 | |
| Gene ID.1 | |
| Uniprot.1 | |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasmic |
| Tissue Expression | Smooth muscle, and tissues containing significant amounts of smooth muscle. |
| Signaling_pathway | |
| Research Areas | |
| Function | Function:Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.,similarity:Belongs to the calponin family.,similarity:Contains 1 CH (calponin-homology) domain.,similarity:Contains 3 calponin-like repeats.,subunit:Part of cGMP kinase signaling complex at least composed of ACTA2/alpha-actin, CNN1/calponin H1, PLN/phospholamban, PRKG1 and ITPR1.,tissue specificity:Smooth muscle, and tissues containing significant amounts of smooth muscle., |
