U-Blot® CaMKII β Rabbit mAb
WR4296 Western blot analysis of lysates from U-87 MG cells, using CaMKII β Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.
Price range: $268.00 through $328.00
Product details
Background:The product of this gene belongs toThe serine/threonine protein kinase family and toThe Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells,The enzyme is composed of four different chains: alpha, beta, gamma, and delta.The product of this gene is a beta chain. It is possible that distinct isoforms of this chain have different cellular localizations and interact differently with calmodulin. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014],
Specifications
| Size | 50μL/100μL |
| Target | CaMKII β |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IF, ELISA |
| MW(Calculated) | 54kD |
| MW(Observed) | 54kD,60kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0491R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | CAMK2B CAM2 CAMK2 CAMKB |
| Protein Name | Calcium/calmodulin-dependent protein kinase type II subunit beta (CaM kinase II subunit beta) (CaMK-II subunit beta) (EC 2.7.11.17) |
| Other Name | |
| Species | Human |
| Gene ID | 816 |
| Uniprot | Q13554, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | P28652, |
| Species.2 | Rat |
| Gene ID.2 | |
| Uniprot.2 | P08413 |
| Cellular Localization | Cytoplasm |
| Tissue Expression | Widely expressed. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain. Expressed in skeletal muscle. |
| Signaling_pathway | Cellular Processes >> Cell growth and death >> Necroptosis;Organismal Systems >> Endocrine system >> Insulin secretion;Organismal Systems >> Endocrine system >> Glucagon signaling pathway;Organismal Systems >> Endocrine system >> GnRH signaling pathway;Organismal Systems >> Endocrine system >> Oxytocin signaling pathway;Organismal Systems >> Endocrine system >> Aldosterone synthesis and secretion;Organismal Systems >> Circulatory system >> Adrenergic signaling in cardiomyocytes;Organismal Systems >> Nervous system >> Cholinergic synapse;Organismal Systems >> Nervous system >> Dopaminergic synapse;Organismal Systems >> Nervous system >> Neurotrophin signaling pathway;Organismal Systems >> Sensory system >> Inflammatory mediator regulation of TRP channels;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: specific types >> Glioma;Human Diseases >> Neurodegenerative disease >> Parkinson disease;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases;Environmental Information Processing >> Signal transduction >> ErbB signaling pathway;Environmental Information Processing >> Signal transduction >> Wnt signaling pathway;Environmental Information Processing >> Signal transduction >> HIF-1 signaling pathway;Environmental Information Processing >> Signal transduction >> Calcium signaling pathway;Environmental Information Processing >> Signal transduction >> cAMP signaling pathway |
| Research Areas | >>ErbB signaling pathway; / >>Calcium signaling pathway; / >>cAMP signaling pathway; / >>HIF-1 signaling pathway; / >>Oocyte meiosis; / >>Necroptosis; / >>Adrenergic signaling in cardiomyocytes; / >>Wnt signaling pathway; / >>Axon guidance; / >>Circadian entrainment; / >>Long-term potentiation; / >>Neurotrophin signaling pathway; / >>Cholinergic synapse; / >>Dopaminergic synapse; / >>Olfactory transduction; / >>Inflammatory mediator regulation of TRP channels; / >>Insulin secretion; / >>GnRH signaling pathway; / >>Melanogenesis; / >>Oxytocin signaling pathway; / >>Glucagon signaling pathway; / >>Aldosterone synthesis and secretion; / >>Cushing syndrome; / >>Gastric acid secretion; / >>Parkinson disease; / >>Pathways of neurodegeneration - multiple diseases; / >>Amphetamine addiction; / >>Tuberculosis; / >>Pathways in cancer; / >>Proteoglycans in cancer; / >>Glioma; / >>Diabetic cardiomyopathy; / >>Lipid and atherosclerosis |
| Function | Alternative products:The variable region of the CAMK2B protein is encoded by at least 7 exons (V1 to V7). Alternative splicing within this region gives rise to CAMK2B isoforms,Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Autophosphorylation of CAMK2 plays an important role in the regulation of the kinase activity.,Function:CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses, it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.,similarity:Contains 1 protein kinase domain.,subunit:CAMK2 is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. Interacts with SYNGAP1 and CAMK2N2 (By similarity). Interacts with MPDZ.,tissue specificity:Widely expressed. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain., |
Please login to post questions
Related products
