SKU: WP11407 Categories: ,

CARD 14 Polyclonal Antibody

Price range: $268.00 through $328.00

SKU: WP11407-100
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Product details

Background:

This gene encodes a caspase recruitment domain-containing protein that is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. Members of this protein family are scaffold proteins that are involved in a diverse array of cellular processes including cellular adhesion, signal transduction and cell polarity control. This protein has been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2012],

Specifications

Size50μL/100μL
TargetCARD 14
ReactivityHuman, Mouse
ApplicationsIHC, IF, ELISA
MW(Calculated)
MW(Observed)110kD
Host SpeciesRabbit
IsotypeIgG
Conjugate/ModificationUnmodified
Recommended Dilution RatioIHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:40000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenThe antiserum was produced against synthesized peptide derived from human CAR14. AA range:291-340
SpecificityCARD 14 Polyclonal Antibody detects endogenous levels of CARD 14 protein.
Gene NameCARD14
Protein NameCaspase recruitment domain-containing protein 14
Other NameCARD14; / CARMA2; / Caspase recruitment domain-containing protein 14; / CARD-containing MAGUK protein 2; / Carma 2
SpeciesHuman
Gene ID79092
UniprotQ9BXL6,
Species.1Mouse
Gene ID.1170720
Uniprot.1Q99KF0
Species.2
Gene ID.2
Uniprot.2
Cellular Localization[Isoform 1]: Cytoplasm .; [Isoform 2]: Cytoplasm .; [Isoform 3]: Cytoplasm .
Tissue ExpressionIsoform 1 is detected in placenta and epidermal keratinocytes (PubMed:22521418). Isoform 2 is detected in leukocytes and fetal brain (PubMed:22521418).
Signaling_pathwayEnvironmental Information Processing >> Signal transduction >> NF-kappa B signaling pathway
Research Areas>>NF-kappa B signaling pathway
FunctionCaution:Supposed to contain a SH3 domain which is not detected by PROSITE, Pfam or SMART.,Function:Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10.,similarity:Contains 1 CARD domain.,similarity:Contains 1 guanylate kinase-like domain.,similarity:Contains 1 PDZ (DHR) domain.,subunit:CARD14 and BCL10 bind to each other by CARD-CARD interaction.,tissue specificity:Expressed in placenta. Also detected in HeLa S3 cells, but not in the other cancer cell lines tested.,
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