Caspase-6 Polyclonal Antibody
Ucallm_WP11427-50
Price range: $268.00 through $328.00
Product details
Background:
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family of enzymes. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic acid residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspases 7, 8 and 10, and is thought to function as a downstream enzyme in the caspase activation cascade. Alternative splicing of this gene results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Oct 2015],
Specifications
| Size | 50μL/100μL |
| Target | Caspase-6 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 35kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:20000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Caspase 6. AA range:223-272 |
| Specificity | Caspase-6 Polyclonal Antibody detects endogenous levels of Caspase-6 protein. |
| Gene Name | CASP6 |
| Protein Name | Caspase6 |
| Other Name | CASP6; / MCH2; / Caspase-6; / CASP-6; / Apoptotic protease Mch-2 |
| Species | Human |
| Gene ID | 839 |
| Uniprot | P55212, |
| Species.1 | Mouse |
| Gene ID.1 | 12368 |
| Uniprot.1 | O08738, |
| Species.2 | Rat |
| Gene ID.2 | |
| Uniprot.2 | O35397 |
| Cellular Localization | Cytoplasm . Nucleus . |
| Tissue Expression | Lung,Lymphocyte,T-cell, |
| Signaling_pathway | Cellular Processes >> Cell growth and death >> Apoptosis |
| Research Areas | >>Apoptosis; / >>Lipid and atherosclerosis |
| Function | Catalytic activity:Strict requirement for Asp at position P1 and has a preferred cleavage sequence of Val-Glu-His-Asp-|-.,enzyme regulation:Activation is suppressed by phosphorylation at Ser-257.,Function:Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death.,PTM:Cleavages by caspase-3, caspase-8 or -10 generate the two active subunits.,similarity:Belongs to the peptidase C14A family.,subunit:Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 11 kDa (p11) subunit., |
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