U-Blot® Cathepsin B Rabbit mAb
Ucallm_WR4958-50
Price range: $268.00 through $328.00
Product details
Background:This gene encodes a member of the C1 family of peptidases. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate multiple protein products. These products include the cathepsin B light and heavy chains, which can dimerize to form the double chain form of the enzyme. This enzyme is a lysosomal cysteine protease with both endopeptidase and exopeptidase activity that may play a role in protein turnover. It is also known as amyloid precursor protein secretase and is involved in the proteolytic processing of amyloid precursor protein (APP). Incomplete proteolytic processing of APP has been suggested to be a causative factor in Alzheimer's disease, the most common cause of dementia. Overexpression of the encoded protein has been associated with esophageal adenocarcinoma and other tumors.
Specifications
| Size | 50μL/100μL |
| Target | Cathepsin B |
| Reactivity | Human |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 38kD |
| MW(Observed) | 38kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1181R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | CTSB CPSB |
| Protein Name | Cathepsin B |
| Other Name | CTSB; / CPSB; / Cathepsin B; / APP secretase; / APPS; / Cathepsin B1; / [Cleaved into: Cathepsin B light chain; / Cathepsin B heavy chain] |
| Species | Human |
| Gene ID | 1508 |
| Uniprot | P07858, |
| Species.1 | Mouse |
| Gene ID.1 | 13030 |
| Uniprot.1 | P10605, |
| Species.2 | Rat |
| Gene ID.2 | |
| Uniprot.2 | P00787 |
| Cellular Localization | Lysosome . Melanosome . Secreted, extracellular space . Apical cell membrane; Peripheral membrane protein; Extracellular side . Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065). Localizes to the lumen of thyroid follicles and to the apical membrane of thyroid epithelial cells (By similarity). . |
| Tissue Expression | Expressed in the stratum spinosum of the epidermis. Weak expression is detected in the stratum granulosum. |
| Signaling_pathway | Cellular Processes >> Transport and catabolism >> Lysosome;Cellular Processes >> Transport and catabolism >> Autophagy - animal;Cellular Processes >> Cell growth and death >> Apoptosis;Organismal Systems >> Immune system >> NOD-like receptor signaling pathway;Organismal Systems >> Immune system >> Antigen processing and presentation |
| Research Areas | >>Autophagy - animal; / >>Lysosome; / >>Apoptosis; / >>Antigen processing and presentation; / >>NOD-like receptor signaling pathway; / >>Renin secretion |
| Function | Catalytic activity:Hydrolysis of proteins with broad specificity for peptide bonds. Preferentially cleaves -Arg-Arg-|-Xaa bonds in small molecule substrates (thus differing from cathepsin L). In addition to being an endopeptidase, shows peptidyl-dipeptidase activity, liberating C-terminal dipeptides.,Function:Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.,similarity:Belongs to the peptidase C1 family.,subcellular location:Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,subunit:Dimer of a heavy chain and a light chain cross-linked by a disulfide bond., |
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