CD11b Polyclonal Antibody
Ucallm_WP15858-50
Price range: $268.00 through $328.00
Product details
Background:
integrin subunit alpha M(ITGAM) Homo sapiens This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009],
Specifications
| Size | 50μL/100μL |
| Target | CD11b |
| Reactivity | Human, Mouse |
| Applications | IHC, IF, mult-IHC, ELISA |
| MW(Calculated) | |
| MW(Observed) | |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:50-200; ELISA 1:10000-20000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthetic peptide from human protein at AA range: 280-360 |
| Specificity | The antibody detects endogenous CD11b |
| Gene Name | ITGAM CD11B CR3A |
| Protein Name | Integrin alpha-M (CD11 antigen-like family member B) (CR-3 alpha chain) (Cell surface glycoprotein MAC-1 subunit alpha) (Leukocyte adhesion receptor MO1) (Neutrophil adherence receptor) (CD antigen CD11b) |
| Other Name | Integrin alpha-M; / CD11 antigen-like family member B; / CR-3 alpha chain; / Cell surface glycoprotein MAC-1 subunit alpha; / Leukocyte adhesion receptor MO1; / Neutrophil adherence receptor; / CD antigen CD11b |
| Species | Human |
| Gene ID | 3684 |
| Uniprot | P11215, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | P05555 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein . Membrane raft; Single-pass type I membrane protein . |
| Tissue Expression | Predominantly expressed in monocytes and granulocytes (PubMed:1346576). Expressed in neutrophils (at protein level) (PubMed:21193407). |
| Signaling_pathway | Cellular Processes >> Transport and catabolism >> Phagosome;Cellular Processes >> Cell motility >> Regulation of actin cytoskeleton;Organismal Systems >> Immune system >> Hematopoietic cell lineage;Organismal Systems >> Immune system >> Complement and coagulation cascades;Organismal Systems >> Immune system >> Neutrophil extracellular trap formation;Organismal Systems >> Immune system >> Leukocyte transendothelial migration;Human Diseases >> Cancer: overview >> Transcriptional misregulation in cancer;Human Diseases >> Cancer: specific types >> Acute myeloid leukemia;Environmental Information Processing >> Signal transduction >> Rap1 signaling pathway;Environmental Information Processing >> Signaling molecules and interaction >> Cell adhesion molecules |
| Research Areas | >>Rap1 signaling pathway; / >>Phagosome; / >>Cell adhesion molecules; / >>Complement and coagulation cascades; / >>Neutrophil extracellular trap formation; / >>Hematopoietic cell lineage; / >>Leukocyte transendothelial migration; / >>Regulation of actin cytoskeleton; / >>Pertussis; / >>Legionellosis; / >>Leishmaniasis; / >>Amoebiasis; / >>Staphylococcus aureus infection; / >>Tuberculosis; / >>Transcriptional misregulation in cancer; / >>Acute myeloid leukemia |
| Function | Disease:Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.,Domain:The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.,Function:Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.,similarity:Belongs to the integrin alpha chain family.,similarity:Contains 1 VWFA domain.,similarity:Contains 7 FG-GAP repeats.,subunit:Heterodimer of an alpha and a beta subunit. Alpha-M associates with beta-2.,tissue specificity:Predominantly expressed in monocytes and granulocytes., |
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