SKU: WP11484 Categories: ,

CD20 Polyclonal Antibody

Price range: $268.00 through $328.00

SKU: WP11484-100
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Product details

Background:

This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq, Jul 2008],

Specifications

Size50μL/100μL
TargetCD20
ReactivityHuman, Mouse
ApplicationsWB, ELISA
MW(Calculated)
MW(Observed)33kD
Host SpeciesRabbit
IsotypeIgG
Conjugate/ModificationUnmodified
Recommended Dilution RatioWB 1:500-1:2000; ELISA 1:40000; Not yet tested in other applications.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenThe antiserum was produced against synthesized peptide derived from human CD20. AA range:181-230
SpecificityCD20 Polyclonal Antibody detects endogenous levels of CD20 protein.
Gene NameMS4A1
Protein NameB-lymphocyte antigen CD20
Other NameMS4A1; / CD20; / B-lymphocyte antigen CD20; / B-lymphocyte surface antigen B1; / Bp35; / Leukocyte surface antigen Leu-16; / Membrane-spanning 4-domains subfamily A member 1; / CD antigen CD20
SpeciesHuman
Gene ID931
UniprotP11836,
Species.1Mouse
Gene ID.112482
Uniprot.1P19437
Species.2
Gene ID.2
Uniprot.2
Cellular LocalizationCell membrane; Multi-pass membrane protein . Cell membrane; Lipid-anchor . Constitutively associated with membrane rafts. .
Tissue ExpressionExpressed on B-cells.
Signaling_pathwayOrganismal Systems >> Immune system >> Hematopoietic cell lineage
Research Areas>>Hematopoietic cell lineage
FunctionCaution:Epitope 1, mapped in PubMed:16785532, is predicted to be buried in the membrane. Its accessibility to the extracellular space, and thus to antibody recognition, is not explained.,Function:This protein may be involved in the regulation of B-cell activation and proliferation.,online information:CD20 entry,pharmaceutical:Monoclonal antibodies(mAb) against CD20 are used to treat B-cell non-Hodgkin's lymphoma (NHL). These antibodies include Rituximab (Mabthera), Britumomab (Zevalin) and Tositumomab (Bexxar). CD20 engaged by mAb can generate transmembrane signals capable of directly controlling cell growth and triggering cell death in certain tumors. Alternatively, mAb can mediate complement-dependent cytotoxicity.,PTM:Phosphorylated. Might be functionally regulated by protein kinase(s).,similarity:Belongs to the MS4A family.,tissue specificity:Expressed on B-cells.,
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