Name: CD284 Polyclonal Antibody
SKU: WP11490
Price: 268 USD
Availability: InStock

Product details

Background:

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. Mutations in this gene have been associated with differences in LPS responsiveness. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012],

Specifications

Size	50μL/100μL
Target	CD284
Reactivity	Human, Mouse
Applications	IF, WB, IHC, ELISA
MW(Calculated)
MW(Observed)	95kD
Host Species	Rabbit
Isotype	IgG
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IF 1:50-200; WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000; Not yet tested in other applications; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration	1 mg/ml
Clonality	Polyclonal
Clone Number
Immunogen	The antiserum was produced against synthesized peptide derived from human CD284. AA range:392-441
Specificity	CD284 Polyclonal Antibody detects endogenous levels of CD284 protein.
Gene Name	TLR4
Protein Name	Toll-like receptor 4
Other Name	TLR4; / Toll-like receptor 4; / hToll; / CD antigen CD284
Species	Human
Gene ID	7099
Uniprot	O00206,
Species.1	Mouse
Gene ID.1	21898
Uniprot.1	Q9QUK6
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Cell membrane; Single-pass type I membrane protein . Early endosome . Cell projection, ruffle . Upon complex formation with CD36 and TLR6, internalized through dynamin-dependent endocytosis (PubMed:20037584). Colocalizes with RFTN1 at cell membrane and then together with RFTN1 moves to endosomes, upon lipopolysaccharide stimulation. .
Tissue Expression	Highly expressed in placenta, spleen and peripheral blood leukocytes (PubMed:9435236, PubMed:9237759). Detected in monocytes, macrophages, dendritic cells and several types of T-cells (PubMed:9237759, PubMed:27022195).
Signaling_pathway	Cellular Processes >> Transport and catabolism >> Phagosome;Cellular Processes >> Cell growth and death >> Necroptosis;Organismal Systems >> Immune system >> Neutrophil extracellular trap formation;Organismal Systems >> Immune system >> Toll-like receptor signaling pathway;Organismal Systems >> Immune system >> NOD-like receptor signaling pathway;Human Diseases >> Cancer: overview >> PD-L1 expression and PD-1 checkpoint pathway in cancer;Human Diseases >> Immune disease >> Rheumatoid arthritis;Human Diseases >> Immune disease >> Inflammatory bowel disease;Environmental Information Processing >> Signal transduction >> NF-kappa B signaling pathway;Environmental Information Processing >> Signal transduction >> HIF-1 signaling pathway;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway
Research Areas	>>NF-kappa B signaling pathway; / >>HIF-1 signaling pathway; / >>Phagosome; / >>PI3K-Akt signaling pathway; / >>Necroptosis; / >>Neutrophil extracellular trap formation; / >>Toll-like receptor signaling pathway; / >>NOD-like receptor signaling pathway; / >>Alcoholic liver disease; / >>Pathogenic Escherichia coli infection; / >>Shigellosis; / >>Salmonella infection; / >>Pertussis; / >>Legionellosis; / >>Yersinia infection; / >>Leishmaniasis; / >>Chagas disease; / >>Malaria; / >>Toxoplasmosis; / >>Amoebiasis; / >>Tuberculosis; / >>Hepatitis B; / >>Measles; / >>Influenza A; / >>Human immunodeficiency virus 1 infection; / >>Coronavirus disease - COVID-19; / >>Proteoglycans in cancer; / >>PD-L1 expression and PD-1 checkpoint pathway in cancer; / >>Inflammatory bowel disease; / >>Rheumatoid arthritis; / >>Lipid and atherosclerosis
Function	Disease:Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.,Domain:The TIR domain mediates interaction with NOX4.,Function:Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.,polymorphism:Allele TLR4*B (Gly-299, Ile-399) is associated with a blunted response to inhaled LPS.,PTM:N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.,similarity:Belongs to the Toll-like receptor family.,similarity:Contains 1 TIR domain.,similarity:Contains 21 LRR (leucine-rich) repeats.,subunit:Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, LY96 and TLR4. Interacts with LY96 via the extracellular domain. Interacts with MYD88 and TIRAP via their respective TIR domains. Interacts with NOX4.,tissue specificity:Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.,