Price range: $268.00 through $328.00
Background:
This gene encodes a protein that contains a mono-ADP-ribosylation (ART) motif. It is a member of the ADP-ribosyltransferase gene family but enzymatic activity has not been demonstrated experimentally. Antigens of the Dombrock blood group system are located on the gene product, which is glycosylphosphatidylinosotol-anchored to the erythrocyte membrane. Allelic variants, some of which lead to adverse transfusion reactions, are known. [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | CD297 |
| Reactivity | Human, Mouse |
| Applications | IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:50-200; ELISA 1:10000-20000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthetic peptide from human protein at AA range: 181-230 |
| Specificity | The antibody detects endogenous CD297 |
| Gene Name | ART4 DO DOK1 |
| Protein Name | Ecto-ADP-ribosyltransferase 4 (ADP-ribosyltransferase C2 and C3 toxin-like 4) (ARTC4) (Dombrock blood group carrier molecule) (Mono(ADP-ribosyl)transferase 4) (NAD(P)(+)--arginine ADP-ribosyltransferase 4) (CD antigen CD297) |
| Other Name | Ecto-ADP-ribosyltransferase 4; / ADP-ribosyltransferase C2 and C3 toxin-like 4; / ARTC4; / Dombrock blood group carrier molecule; / Mono; / ADP-ribosyl; / transferase 4; / NAD; / P; / +; / --arginine ADP-ribosyltransferase 4; / CD antigen CD297 |
| Species | Human |
| Gene ID | 420 |
| Uniprot | Q93070, |
| Species.1 | Mouse |
| Gene ID.1 | 109978 |
| Uniprot.1 | Q9CRA0 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cell membrane; Lipid-anchor, GPI-anchor. |
| Tissue Expression | Expressed in spleen and T-cells. |
| Signaling_pathway | |
| Research Areas | |
| Function | Catalytic activity:NAD(+) + protein-L-arginine = nicotinamide + N(omega)-(ADP-D-ribosyl)-protein-L-arginine.,Catalytic activity:NADP(+) + protein-L-arginine = nicotinamide + N(omega)-((2'-phospho-ADP)-D-ribosyl)-protein-L-arginine.,online information:Blood group antigen gene mutation database,polymorphism:DO is responsible for the Dombrock blood group system. The molecular basis of the Do(a)/Do(b) blood group antigen is a single variation in position 265; Asn-265 corresponds to Do(a) and Asp-265 to Do(b). It is also responsible for the antigens Gregory [Gy(a)], Holley [Hy] and Joseph [Jo(a)].,similarity:Belongs to the Arg-specific ADP-ribosyltransferase family.,tissue specificity:Expressed in spleen and T-cells., |
