Name: CD4 Monoclonal Antibody
SKU: WM0098
Price: 268 USD
Availability: InStock

Product details

Background:
This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010],

Specifications

Target	CD4
Reactivity	Human
Applications	FC, ELISA
MW(Calculated)
MW(Observed)
Host Species	Mouse
Isotype
Conjugate/Modification	Unmodified
Modification
Recommended Dilution Ratio	Flow Cyt 1:200-1:400; ELISA 1:10000; Not yet tested in other applications;
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Purification	Affinity purification
Purity
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	20D10
Immunogen	Purified recombinant fragment of human CD4 expressed in E. Coli.
Sequence
Specificity	CD4 Monoclonal Antibody detects endogenous levels of CD4 protein.
Gene Name	CD4
Protein Name	T-cell surface glycoprotein CD4
Other Name	CD4; / T-cell surface glycoprotein CD4; / T-cell surface antigen T4/Leu-3; / CD antigen CD4
Species	Human
Gene ID-1	920
Uniprot	P01730,
Species.1	Mouse
Gene ID-2
Uniprot.1	P06332
Species.2
Gene ID-3
Uniprot.2
Organism-4
Gene ID-4
SwissProt-4
Background	This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010],
Cellular Localization	Cell membrane; Single-pass type I membrane protein . Localizes to lipid rafts (PubMed:12517957, PubMed:9168119). Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
Tissue Expression	Highly expressed in T-helper cells. The presence of CD4 is a hallmark of T-helper cells which are specialized in the activation and growth of cytotoxic T-cells, regulation of B cells, or activation of phagocytes. CD4 is also present in other immune cells such as macrophages, dendritic cells or NK cells.
Signaling_pathway	Organismal Systems >> Immune system >> Hematopoietic cell lineage;Organismal Systems >> Immune system >> Antigen processing and presentation;Organismal Systems >> Immune system >> T cell receptor signaling pathway;Organismal Systems >> Immune system >> Th1 and Th2 cell differentiation;Organismal Systems >> Immune system >> Th17 cell differentiation;Human Diseases >> Cancer: overview >> PD-L1 expression and PD-1 checkpoint pathway in cancer;Human Diseases >> Immune disease >> Primary immunodeficiency;Environmental Information Processing >> Signaling molecules and interaction >> Cytokine-cytokine receptor interaction;Environmental Information Processing >> Signaling molecules and interaction >> Cell adhesion molecules
Research Areas	>>Viral life cycle - HIV-1; / >>Cytokine-cytokine receptor interaction; / >>Cell adhesion molecules; / >>Antigen processing and presentation; / >>Hematopoietic cell lineage; / >>Th1 and Th2 cell differentiation; / >>Th17 cell differentiation; / >>T cell receptor signaling pathway; / >>Yersinia infection; / >>Human T-cell leukemia virus 1 infection; / >>Human immunodeficiency virus 1 infection; / >>PD-L1 expression and PD-1 checkpoint pathway in cancer; / >>Primary immunodeficiency
Function	Function:Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.,miscellaneous:Primary receptor for HIV-1.,online information:CD4 entry,PTM:Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.,similarity:Contains 1 Ig-like V-type (immunoglobulin-like) domain.,similarity:Contains 3 Ig-like C2-type (immunoglobulin-like) domains.,subcellular location:Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.,subunit:Associates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to the cell membrane, is part of P4HB/PDI-CD4-CXCR4-gp120 complex. Interacts with HIV-1 Envelope polyprotein gp160 and protein Vpu. Interacts with Human Herpes virus 7 capsid proteins.,
RRID

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CD4 Monoclonal Antibody

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CD4 Monoclonal Antibody

Product details

Specifications

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