CD4 [PTR2055] mouse mAb
Ucallm_WM1259-50
Price range: $268.00 through $328.00
Product details
Background:
CD4 molecule(CD4) Homo sapiens This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010],
Specifications
| Size | 50μL/100μL |
| Target | CD4 |
| Reactivity | Human |
| Applications | WB, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 51kD |
| Host Species | Mouse |
| Isotype | IgG1,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-2000; IF 1:100-500; ELISA 1:1000-5000 |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein G affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Monoclonal |
| Clone Number | RMMR2055 |
| Immunogen | Synthesized peptide derived from human CD4. AA range: 1-100 |
| Specificity | This antibody detects endogenous levels of CD4 protein. |
| Gene Name | CD4 |
| Protein Name | T-cell surface glycoprotein CD4 (T-cell surface antigen T4/Leu-3) (CD antigen CD4) |
| Other Name | T-cell surface glycoprotein CD4; / T-cell surface antigen T4/Leu-3; / CD antigen CD4 |
| Species | Human |
| Gene ID | 920 |
| Uniprot | P01730, |
| Species.1 | Mouse |
| Gene ID.1 | 12504 |
| Uniprot.1 | P06332, |
| Species.2 | Rat |
| Gene ID.2 | 24932 |
| Uniprot.2 | P05540 |
| Cellular Localization | Membranous |
| Tissue Expression | Highly expressed in T-helper cells. The presence of CD4 is a hallmark of T-helper cells which are specialized in the activation and growth of cytotoxic T-cells, regulation of B cells, or activation of phagocytes. CD4 is also present in other immune cells such as macrophages, dendritic cells or NK cells. |
| Signaling_pathway | Organismal Systems >> Immune system >> Hematopoietic cell lineage;Organismal Systems >> Immune system >> Antigen processing and presentation;Organismal Systems >> Immune system >> T cell receptor signaling pathway;Organismal Systems >> Immune system >> Th1 and Th2 cell differentiation;Organismal Systems >> Immune system >> Th17 cell differentiation;Human Diseases >> Cancer: overview >> PD-L1 expression and PD-1 checkpoint pathway in cancer;Human Diseases >> Immune disease >> Primary immunodeficiency;Environmental Information Processing >> Signaling molecules and interaction >> Cytokine-cytokine receptor interaction;Environmental Information Processing >> Signaling molecules and interaction >> Cell adhesion molecules |
| Research Areas | |
| Function | Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.; (Microbial infection) Primary receptor for human immunodeficiency virus-1 (HIV-1) . Down-regulated by HIV-1 Vpu . Acts as a receptor for Human Herpes virus 7/HHV-7 . |
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