Name: CD86 Monoclonal Antibody
SKU: WM0107
Price: 268 USD
Availability: InStock

Product details

Background:
This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011],

Specifications

Size	50μL/100μL
Target	CD86
Reactivity	Human
Applications	WB, ELISA
MW(Calculated)	60-80kD
MW(Observed)
Host Species	Mouse
Isotype
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:500-1:2000; ELISA 1:10000; Not yet tested in other applications.
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification	Affinity purification
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	7E3
Immunogen	Purified recombinant fragment of human CD86 expressed in E. Coli.
Specificity	CD86 Monoclonal Antibody detects endogenous levels of CD86 protein.
Gene Name	CD86
Protein Name	T-lymphocyte activation antigen CD86
Other Name	CD86; / CD28LG2; / T-lymphocyte activation antigen CD86; / Activation B7-2 antigen; / B70; / BU63; / CTLA-4 counter-receptor B7.2; / FUN-1; / CD antigen CD86
Species	Human
Gene ID	942
Uniprot	P42081,
Species.1	Mouse
Gene ID.1
Uniprot.1	P42082
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Cell membrane; Single-pass type I membrane protein.
Tissue Expression	Expressed by activated B-lymphocytes and monocytes.
Signaling_pathway	Organismal Systems >> Immune system >> Toll-like receptor signaling pathway;Organismal Systems >> Immune system >> Intestinal immune network for IgA production;Human Diseases >> Cancer: overview >> Transcriptional misregulation in cancer;Human Diseases >> Immune disease >> Systemic lupus erythematosus;Human Diseases >> Immune disease >> Rheumatoid arthritis;Human Diseases >> Immune disease >> Autoimmune thyroid disease;Human Diseases >> Immune disease >> Allograft rejection;Human Diseases >> Immune disease >> Graft-versus-host disease;Environmental Information Processing >> Signaling molecules and interaction >> Cell adhesion molecules
Research Areas	>>Cell adhesion molecules; / >>Toll-like receptor signaling pathway; / >>Intestinal immune network for IgA production; / >>Type I diabetes mellitus; / >>Kaposi sarcoma-associated herpesvirus infection; / >>Transcriptional misregulation in cancer; / >>Autoimmune thyroid disease; / >>Systemic lupus erythematosus; / >>Rheumatoid arthritis; / >>Allograft rejection; / >>Graft-versus-host disease; / >>Viral myocarditis
Function	Function:Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.,online information:CD86 entry,PTM:Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.,similarity:Contains 1 Ig-like C2-type (immunoglobulin-like) domain.,similarity:Contains 1 Ig-like V-type (immunoglobulin-like) domain.,subunit:Interacts with MARCH8. Interacts with human herpesvirus 8 MIR2 protein (Probable). Interacts with adenovirus subgroup B fiber proteins and acts as a receptor for these viruses.,tissue specificity:Expressed by activated B-lymphocytes and monocytes.,