U-Blot® CD86 Rabbit mAb
WR4005 J774A.1 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with primary antibody(1:1000). The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.
Price range: $268.00 through $328.00
Product details
Background:This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011],
Specifications
| Size | 50μL/100μL |
| Target | CD86 |
| Reactivity | Mouse |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 60-80kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1000; WB 1:500-5000; IF 1:200-1000; ELISA 1:5000-20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0048R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | CD86 CD28LG2 |
| Protein Name | CD86 |
| Other Name | T-lymphocyte activation antigen CD86; / Activation B7-2 antigen; / B70; / BU63; / CTLA-4 counter-receptor B7.2; / FUN-1; / CD antigen CD86 |
| Species | Human |
| Gene ID | 942 |
| Uniprot | P42081, |
| Species.1 | Mouse |
| Gene ID.1 | 12524 |
| Uniprot.1 | P42082 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Membranous |
| Tissue Expression | |
| Signaling_pathway | Organismal Systems >> Immune system >> Toll-like receptor signaling pathway;Organismal Systems >> Immune system >> Intestinal immune network for IgA production;Human Diseases >> Cancer: overview >> Transcriptional misregulation in cancer;Human Diseases >> Immune disease >> Systemic lupus erythematosus;Human Diseases >> Immune disease >> Rheumatoid arthritis;Human Diseases >> Immune disease >> Autoimmune thyroid disease;Human Diseases >> Immune disease >> Allograft rejection;Human Diseases >> Immune disease >> Graft-versus-host disease;Environmental Information Processing >> Signaling molecules and interaction >> Cell adhesion molecules |
| Research Areas | >>Cell adhesion molecules; / >>Toll-like receptor signaling pathway; / >>Intestinal immune network for IgA production; / >>Type I diabetes mellitus; / >>Kaposi sarcoma-associated herpesvirus infection; / >>Transcriptional misregulation in cancer; / >>Autoimmune thyroid disease; / >>Systemic lupus erythematosus; / >>Rheumatoid arthritis; / >>Allograft rejection; / >>Graft-versus-host disease; / >>Viral myocarditis |
| Function | Function:Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.,online information:CD86 entry,PTM:Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.,similarity:Contains 1 Ig-like C2-type (immunoglobulin-like) domain.,similarity:Contains 1 Ig-like V-type (immunoglobulin-like) domain.,subunit:Interacts with MARCH8. Interacts with human herpesvirus 8 MIR2 protein (Probable). Interacts with adenovirus subgroup B fiber proteins and acts as a receptor for these viruses.,tissue specificity:Expressed by activated B-lymphocytes and monocytes., |
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