U-Blot® CD99 Rabbit mAb
WR4184 Western blot analysis of lysates from Hela cells, using NDUFS3 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.
Price range: $268.00 through $328.00
Product details
Background:The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. There is a related pseudogene located immediately adjacent to this locus. [provided by RefSeq, Mar 2016],
Specifications
| Size | 50μL/100μL |
| Target | CD99 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 19kD |
| MW(Observed) | 30kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:500-1:2000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200, / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0453R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | CD99 MIC2 MIC2X MIC2Y |
| Protein Name | CD99 |
| Other Name | CD99 antigen; / 12E7; / E2 antigen; / Protein MIC2; / T-cell surface glycoprotein E2; / CD antigen CD99 |
| Species | Human |
| Gene ID | 4722 |
| Uniprot | P14209, |
| Species.1 | Mouse |
| Gene ID.1 | 68349 |
| Uniprot.1 | Q9DCT2 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Membrane |
| Tissue Expression | Brain,Cajal-Retzius cell,Pituitary,Skin,Stomach mucosa,Uter |
| Signaling_pathway | Human Diseases >> Neurodegenerative disease >> Alzheimer disease;Human Diseases >> Neurodegenerative disease >> Parkinson disease;Human Diseases >> Neurodegenerative disease >> Amyotrophic lateral sclerosis;Human Diseases >> Neurodegenerative disease >> Huntington disease;Human Diseases >> Neurodegenerative disease >> Prion disease;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases |
| Research Areas | >>Oxidative phosphorylation; / >>Metabolic pathways; / >>Thermogenesis; / >>Retrograde endocannabinoid signaling; / >>Non-alcoholic fatty liver disease; / >>Alzheimer disease; / >>Parkinson disease; / >>Amyotrophic lateral sclerosis; / >>Huntington disease; / >>Prion disease; / >>Pathways of neurodegeneration - multiple diseases; / >>Chemical carcinogenesis - reactive oxygen species; / >>Diabetic cardiomyopathy |
| Function | Function:Involved in T-cell adhesion processes. It is involved in spontaneous rosette formation with erythrocytes.,miscellaneous:The gene encoding for this protein is located in the pseudoautosomal region 1 (PAR1) of X and Y chromosomes.,PTM:Extensively O-glycosylated.,similarity:Belongs to the CD99 family., |
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