Price range: $268.00 through $328.00
Background:cell division cycle 25C(CDC25C) Homo sapiens This gene encodes a conserved protein that plays a key role in the regulation of cell division. The encoded protein directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described. [provided by RefSeq, Dec 2015],
| Size | 50μL/100μL |
| Target | Cdc25C |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 53kD |
| MW(Observed) | 60kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:1000-1:4000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0669R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | CDC25C |
| Protein Name | M-phase inducer phosphatase 3 |
| Other Name | CDC25C; / M-phase inducer phosphatase 3; / Dual specificity phosphatase Cdc25C |
| Species | Human |
| Gene ID | 6932 |
| Uniprot | P30307, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | P48967 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus |
| Tissue Expression | Predominantly expressed in T-cells. Also detected in proliferating intestinal epithelial cells and in the basal epithelial cells of mammary gland epithelium. |
| Signaling_pathway | Cellular Processes >> Cellular community - eukaryotes >> Adherens junction;Cellular Processes >> Cellular community - eukaryotes >> Signaling pathways regulating pluripotency of stem cells;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: specific types >> Colorectal cancer;Human Diseases >> Cancer: specific types >> Hepatocellular carcinoma;Human Diseases >> Cancer: specific types >> Gastric cancer;Human Diseases >> Cancer: specific types >> Thyroid cancer;Human Diseases >> Cancer: specific types >> Acute myeloid leukemia;Human Diseases >> Cancer: specific types >> Basal cell carcinoma;Human Diseases >> Cancer: specific types >> Prostate cancer;Human Diseases >> Cancer: specific types >> Endometrial cancer;Human Diseases >> Cancer: specific types >> Breast cancer;Environmental Information Processing >> Signal transduction >> Wnt signaling pathway;Environmental Information Processing >> Signal transduction >> Hippo signaling pathway |
| Research Areas | >>Wnt signaling pathway; / >>Hippo signaling pathway; / >>Adherens junction; / >>Signaling pathways regulating pluripotency of stem cells; / >>Melanogenesis; / >>Cushing syndrome; / >>Alcoholic liver disease; / >>Salmonella infection; / >>Human papillomavirus infection; / >>Kaposi sarcoma-associated herpesvirus infection; / >>Pathways in cancer; / >>Colorectal cancer; / >>Endometrial cancer; / >>Prostate cancer; / >>Thyroid cancer; / >>Basal cell carcinoma; / >>Acute myeloid leukemia; / >>Breast cancer; / >>Hepatocellular carcinoma; / >>Gastric cancer; / >>Arrhythmogenic right ventricular cardiomyopathy |
| Function | Catalytic activity:Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate.,developmental stage:Expressed predominantly in G2 phase.,Function:Functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It directly dephosphorylates CDC2 and activate its kinase activity.,PTM:Phosphorylated by CHK1 on Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase.,similarity:Belongs to the MPI phosphatase family.,similarity:Contains 1 rhodanese domain.,subunit:Interacts with HIV-1 Vpr, thereby inactivating CDC25C phosphatase activity., |
