Price range: $268.00 through $328.00
Background:
The protein encoded by this gene is a member of the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzyme catalyzes the covalent attachment of ubiquitin to other proteins. This protein is a part of the large multiprotein complex, which is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for the initiation of DNA replication. [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | Cdc34 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 34kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC: 1:100-300; ELISA 1:20000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human CDC34. AA range:121-170 |
| Specificity | Cdc34 Polyclonal Antibody detects endogenous levels of Cdc34 protein. |
| Gene Name | CDC34 |
| Protein Name | Ubiquitin-conjugating enzyme E2 R1 |
| Other Name | CDC34; / UBCH3; / UBE2R1; / Ubiquitin-conjugating enzyme E2 R1; / Ubiquitin-conjugating enzyme E2-32 kDa complementing; / Ubiquitin-conjugating enzyme E2-CDC34; / Ubiquitin-protein ligase R1 |
| Species | Human |
| Gene ID | 997 |
| Uniprot | P49427, |
| Species.1 | Mouse |
| Gene ID.1 | 216150 |
| Uniprot.1 | Q8CFI2 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm. Nucleus. The phosphorylation of the C-terminal tail plays an important role in mediating nuclear localization. Colocalizes with beta-tubulin on mitotic spindles in anaphase. |
| Tissue Expression | Expressed in testes during spermatogenesis to regulate repression of cAMP-induced transcription. |
| Signaling_pathway | Genetic Information Processing >> Folding, sorting and degradation >> Ubiquitin mediated proteolysis |
| Research Areas | >>Ubiquitin mediated proteolysis |
| Function | Catalytic activity:ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.,Function:Catalyzes the covalent attachment of ubiquitin to other proteins. May be involved in degradation of katenin.,pathway:Protein modification; protein ubiquitination.,similarity:Belongs to the ubiquitin-conjugating enzyme family.,subunit:Interacts with SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex. When phosphorylated, interacts with beta-TrCP (BTRC)., |
