Price range: $268.00 through $328.00
Background:
This gene encodes a cell division cycle protein with kinase activity that is critical for the G1/S transition. The yeast homolog is also essential for initiation of DNA replication as cell division occurs. Overexpression of this gene product may be associated with neoplastic transformation for some tumors. Multiple alternatively spliced transcript variants that encode the same protein have been detected. [provided by RefSeq, Aug 2008],
| Size | 50μL/100μL |
| Target | Cdc7 |
| Reactivity | Human, Mouse |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 70kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CDC7. AA range:1-50 |
| Specificity | Cdc7 Polyclonal Antibody detects endogenous levels of Cdc7 protein. |
| Gene Name | CDC7 |
| Protein Name | Cell division cycle 7-related protein kinase |
| Other Name | CDC7; / CDC7L1; / Cell division cycle 7-related protein kinase; / CDC7-related kinase; / HsCdc7; / huCdc7 |
| Species | Human |
| Gene ID | 8317 |
| Uniprot | O00311, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q9Z0H0 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus. |
| Tissue Expression | Brain,Embryonal rhabdomyosarcoma,Liver,Testis, |
| Signaling_pathway | Cellular Processes >> Cell growth and death >> Cell cycle |
| Research Areas | >>Cell cycle |
| Function | Alternative products:A number of isoforms may be produced,Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,Function:Seems to phosphorylate critical substrates that regulate the G1/S phase transition and/or DNA replication. Can phosphorylates MCM2 and MCM3.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC7 subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Forms a complex with either DBF4/DBF4A or DBF4B, leading to the activation of the kinase activity., |
