CHSY2 Polyclonal Antibody
Ucallm_WP11632-50
Price range: $268.00 through $328.00
Product details
Background:
catalytic activity:UDP-alpha-D-glucuronate + N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan = UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan., catalytic activity:UDP-N-acetyl-D-galactosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-proteoglycan = UDP + N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-proteoglycan., cofactor:Divalent cations. Highest activities are measured with manganese. Can also utilize cobalt., function:Has both beta-1, 3-glucuronic acid and beta-1, 4-N-acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer., online information:GlycoGene database, PTM:Phosphorylated upon DNA damage, probably by ATM or ATR., similarity:Belongs to the chondroitin N-acetylgalactosaminyltransferase family., subunit:Binds CHSY1., tissue specificity:Ubiquitous. Highly expressed in pancreas, ovary, brain, heart, skeletal muscle, colon, kidney, liver, stomach, small intestine and placenta.,
Specifications
| Size | 50μL/100μL |
| Target | CHSY2 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 85kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:40000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CHSY2. AA range:631-680 |
| Specificity | CHSY2 Polyclonal Antibody detects endogenous levels of CHSY2 protein. |
| Gene Name | CHPF |
| Protein Name | Chondroitin sulfate synthase 2 |
| Other Name | CHPF; / CSS2; / Chondroitin sulfate synthase 2; / Chondroitin glucuronyltransferase 2; / Chondroitin-polymerizing factor; / ChPF; / Glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase II; / N-acetylgalactosaminyl-pro |
| Species | Human |
| Gene ID | 79586 |
| Uniprot | Q8IZ52, |
| Species.1 | Mouse |
| Gene ID.1 | 74241 |
| Uniprot.1 | Q6IQX7 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | [Isoform 1]: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein . Cytoplasm, cytosol .; [Isoform 3]: Cytoplasm, cytosol . Mitochondrion .; [Isoform 2]: Mitochondrion matrix . |
| Tissue Expression | Ubiquitous. Highly expressed in pancreas, ovary, brain, heart, skeletal muscle, colon, kidney, liver, stomach, spleen and placenta.; [Isoform 2]: Expressed in brain, spleen, ovary, testis, lung and peripheral mononuclear cells.; [Isoform 3]: Also ubiquitous. |
| Signaling_pathway | |
| Research Areas | >>Glycosaminoglycan biosynthesis - chondroitin sulfate / dermatan sulfate; / >>Metabolic pathways |
| Function | Catalytic activity:UDP-alpha-D-glucuronate + N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan = UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan.,Catalytic activity:UDP-N-acetyl-D-galactosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-proteoglycan = UDP + N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-proteoglycan.,cofactor:Divalent cations. Highest activities are measured with manganese. Can also utilize cobalt.,Function:Has both beta-1,3-glucuronic acid and beta-1,4-N-acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer.,online information:GlycoGene database,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the chondroitin N-acetylgalactosaminyltransferase family.,subunit:Binds CHSY1.,tissue specificity:Ubiquitous. Highly expressed in pancreas, ovary, brain, heart, skeletal muscle, colon, kidney, liver, stomach, small intestine and placenta., |
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