Price range: $268.00 through $328.00
Background:
This gene is a member of the aggrecan/versican proteoglycan family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Multiple alternatively spliced transcript variants that encode different protein isoforms have been observed in this gene. [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | Aggrecan core protein |
| Reactivity | Human, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 44kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Aggrecan. AA range:320-369 |
| Specificity | Cleaved-Aggrecan (D369) Polyclonal Antibody detects endogenous levels of fragment of activated Aggrecan protein resulting from cleavage adjacent to D369. |
| Gene Name | ACAN AGC1 CSPG1 MSK16 |
| Protein Name | Aggrecan core protein |
| Other Name | ACAN; / AGC1; / CSPG1; / MSK16; / Aggrecan core protein; / Cartilage-specific proteoglycan core protein; / CSPCP; / Chondroitin sulfate proteoglycan core protein 1; / Chondroitin sulfate proteoglycan 1 |
| Species | Human |
| Gene ID | 176 |
| Uniprot | P16112, |
| Species.1 | Mouse |
| Gene ID.1 | 11595 |
| Uniprot.1 | Q61282, |
| Species.2 | Rat |
| Gene ID.2 | 58968 |
| Uniprot.2 | P07897 |
| Cellular Localization | Secreted, extracellular space, extracellular matrix . |
| Tissue Expression | Restricted to cartilages. |
| Signaling_pathway | |
| Research Areas | |
| Function | This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region. |
