CLIC1 Polyclonal Antibody
Ucallm_WP5886-50
Price range: $268.00 through $328.00
Product details
Background:
chloride intracellular channel 1(CLIC1) Homo sapiens Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 1 is a member of the p64 family; the protein localizes principally to the cell nucleus and exhibits both nuclear and plasma membrane chloride ion channel activity. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | CLIC1 |
| Reactivity | Human, Mouse, Rat |
| Applications | IHC, IF |
| MW(Calculated) | |
| MW(Observed) | 30kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:100-200; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthetic Peptide of CLIC1 AA range: 166-216 |
| Specificity | CLIC1 protein(A216) detects endogenous levels of CLIC1 |
| Gene Name | CLIC1 |
| Protein Name | Chloride intracellular channel protein 1 (Chloride channel ABP) (Nuclear chloride ion channel 27) (NCC27) (Regulatory nuclear chloride ion channel protein) (hRNCC) |
| Other Name | Chloride intracellular channel protein 1; / Chloride channel ABP; / Nuclear chloride ion channel 27; / NCC27; / Regulatory nuclear chloride ion channel protein; / hRNCC |
| Species | Human |
| Gene ID | 1192 |
| Uniprot | O00299, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q9Z1Q5, |
| Species.2 | Rat |
| Gene ID.2 | |
| Uniprot.2 | Q6MG61 |
| Cellular Localization | Nucleus . Nucleus membrane; Single-pass membrane protein . Cytoplasm . Cell membrane; Single-pass membrane protein . Mostly in the nucleus including in the nuclear membrane (PubMed:9139710, PubMed:12681486). Small amount in the cytoplasm and the plasma membrane (PubMed:9139710). Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain (PubMed:11940526, PubMed:11551966, PubMed:14613939). . |
| Tissue Expression | Expression is prominent in heart, placenta, liver, kidney and pancreas. |
| Signaling_pathway | |
| Research Areas | |
| Function | Domain:Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion.,Function:Can insert into membranes and form chloride ion channels. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions.,miscellaneous:The protein seems to have very low affinity for glutathion, even though glutathion binding was observed in protein crystals.,PTM:Hydrogen peroxide treatment causes a conformation change, leading to dimerization and formation of an intramolecular disulfide bond between Cys-24 and Cys-59.,similarity:Belongs to the chloride channel CLIC family.,similarity:Contains 1 GST C-terminal domain.,subcellular location:Mostly in the nucleus including in the nuclear membrane. Small amount in the cytoplasm and the plasma membrane. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain.,subunit:Monomer. Homodimer (in vitro). Interacts with TRAPPC2. Dimerization requires a conformation change that leads to the exposure of a large hydrophobic surface. In vivo, this may lead to membrane insertion., |
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