Cubilin Polyclonal Antibody
Ucallm_WP11835-50
Price range: $268.00 through $328.00
Product details
Background:
Cubilin (CUBN) acts as a receptor for intrinsic factor-vitamin B12 complexes. The role of receptor is supported by the presence of 27 CUB domains. Cubulin is located within the epithelium of intestine and kidney. Mutations in CUBN may play a role in autosomal recessive megaloblastic anemia. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | Cubilin |
| Reactivity | Human, Mouse, Rat |
| Applications | IHC, IF, WB, ELISA |
| MW(Calculated) | |
| MW(Observed) | 400kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-2000; IHC 1:100-1:300; ELISA 1:40000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthesized peptide derived from the N-terminal region of human Cubilin. |
| Specificity | Cubilin Polyclonal Antibody detects endogenous levels of Cubilin protein. |
| Gene Name | CUBN |
| Protein Name | Cubilin |
| Other Name | CUBN; / IFCR; / Cubilin; / 460 kDa receptor; / Intestinal intrinsic factor receptor; / Intrinsic factor-cobalamin receptor; / Intrinsic factor-vitamin B12 receptor |
| Species | Human |
| Gene ID | 8029 |
| Uniprot | O60494, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q9JLB4 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Apical cell membrane; Peripheral membrane protein . Cell membrane; Peripheral membrane protein . Membrane, coated pit . Endosome . Lysosome membrane; Peripheral membrane protein . Lacks a transmembrane domain and depends on interaction with AMN for location at the plasma membrane (PubMed:29402915, PubMed:30523278). Colocalizes with AMN and LRP2 in the endocytotic apparatus of epithelial cells (By similarity). . |
| Tissue Expression | Detected in kidney cortex (at protein level) (PubMed:9572993). Expressed in kidney proximal tubule cells, placenta, visceral yolk-sac cells and in absorptive intestinal cells. Expressed in the epithelium of intestine and kidney. |
| Signaling_pathway | |
| Research Areas | >>Vitamin digestion and absorption |
| Function | Disease:Defects in CUBN are a cause of recessive hereditary megaloblastic anemia 1 (MGA1) [MIM:261100]; also referred to as MGA1 Norwegian type or Imerslund-Grasbeck syndrome (I-GS). MGA1 is due to selective malabsorption of vitamin B12. Defects in vitamin B12 absorption lead to impaired function of thymidine synthase. As a consequence DNA synthesis is interrupted. Rapidly dividing cells involved in erythropoiesis are particularly affected.,Domain:The CUB domains 5 to 8 mediate binding to GIF and ALB. CUB domains 1 and 2 mediate interaction with LRP2.,Function:Cotransporter which plays a role in lipoprotein, vitamin and iron metabolism, by facilitating their uptake. Binds to ALB, MB, Kappa and lambda-light chains, TF, hemoglobin, GC, SCGB1A1, APOA1, high density lipoprotein, and the GIF-cobalamin complex. The binding of all ligands required calcium. Serves as important transporter in several absorptive epithelia, including intestine, renal proximal tubules and embryonic yolk sac. Interaction with LRP2 mediates its trafficking throughout vesicles and facilitates the uptake of specific ligands like GC, hemoglobin, ALB, TF and SCGB1A1. Interaction with AMN controls its trafficking to the plasma membrane and facilitates endocytosis of ligands. May play an important role in the development of the peri-implantation embryo through internalization of APOA1 and cholesterol. Binds to LGALS3 at the maternal-fetal interface.,PTM:N-glycosylated.,PTM:The precursor is cleaved by a trans-Golgi proteinase furin. The result is a propeptide cleaved off.,similarity:Contains 27 CUB domains.,similarity:Contains 7 EGF-like domains.,subcellular location:Colocalizes with AMN and LRP2 in the endocytotic apparatus of epithelial cells.,subunit:Interacts with LRP2 in a dual-receptor complex in a calcium-dependent manner. Component of the cubam complex composed of CUBN and AMN. Found in a complex with PID1/PCLI1, LRP1 and CUBNI. Interacts with LRP1 and PID1/PCLI1.,tissue specificity:Expressed in kidney proximal tubule cells, placenta, visceral yolk-sac cells and in absorptive intestinal cells. Expressed in the epithelium of intestine and kidney., |
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