SKU: WP11857 Categories: ,

Cyclin G Polyclonal Antibody

Price range: $268.00 through $328.00

SKU: WP11857-100
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Product details

Background:

The eukaryotic cell cycle is governed by cyclin-dependent protein kinases (CDKs) whose activities are regulated by cyclins and CDK inhibitors. The protein encoded by this gene is a member of the cyclin family and contains the cyclin box. The encoded protein lacks the protein destabilizing (PEST) sequence that is present in other family members. Transcriptional activation of this gene can be induced by tumor protein p53. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008],

Specifications

Size50μL/100μL
TargetCyclin G
ReactivityHuman, Mouse, Rat
ApplicationsWB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)29kD
Host SpeciesRabbit
IsotypeIgG
Conjugate/ModificationUnmodified
Recommended Dilution RatioWB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenThe antiserum was produced against synthesized peptide derived from human Cyclin G. AA range:161-210
SpecificityCyclin G Polyclonal Antibody detects endogenous levels of Cyclin G protein.
Gene NameCCNG1
Protein NameCyclin-G1
Other NameCCNG1; / CCNG; / CYCG1; / Cyclin-G1; / Cyclin-G
SpeciesHuman
Gene ID900
UniprotP51959,
Species.1Mouse
Gene ID.112450
Uniprot.1P51945,
Species.2Rat
Gene ID.225405
Uniprot.2P39950
Cellular LocalizationNucleus . DNA replication foci after DNA damage.
Tissue ExpressionHigh levels in skeletal muscle, ovary, kidney and colon.
Signaling_pathwayCellular Processes >> Cell growth and death >> p53 signaling pathway;Human Diseases >> Cancer: overview >> MicroRNAs in cancer
Research Areas>>p53 signaling pathway; / >>MicroRNAs in cancer
Functiondevelopmental stage:Very low levels in normal cells during G1 phase, which increase as cells enter the S phase and stay high throughout the S and G2/M phases. In breast cancer cells consistent high levels are found throughout the cell cycle.,Function:May play a role in growth regulation. Is associated with G2/M phase arrest in response to DNA damage. May be an intermediate by which p53 mediates its role as an inhibitor of cellular proliferation.,induction:Activated in breast and prostate cancer cells. Activated by actinomycin-D induced DNA damage.,similarity:Belongs to the cyclin family. Cyclin G subfamily.,subcellular location:DNA replication foci after DNA damage.,tissue specificity:High levels in skeletal muscle, ovary, kidney and colon.,
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