SKU: WP11905 Categories: ,

CYP4F2 Polyclonal Antibody

Price range: $268.00 through $328.00

SKU: WP11905-100
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Product details

Background:

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. The enzyme starts the process of inactivating and degrading leukotriene B4, a potent mediator of inflammation. This gene is part of a cluster of cytochrome P450 genes on chromosome 19. Another member of this family, CYP4F11, is approximately 16 kb away. [provided by RefSeq, Jul 2008],

Specifications

Size50μL/100μL
TargetCYP4F2
ReactivityHuman
ApplicationsWB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)60kD
Host SpeciesRabbit
IsotypeIgG
Conjugate/ModificationUnmodified
Recommended Dilution RatioWB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenThe antiserum was produced against synthesized peptide derived from human CYP4F2. AA range:41-90
SpecificityCYP4F2 Polyclonal Antibody detects endogenous levels of CYP4F2 protein.
Gene NameCYP4F2
Protein NameLeukotriene-B(4) omega-hydroxylase 1
Other NameCYP4F2; / Leukotriene-B; / 4; / omega-hydroxylase 1; / CYPIVF2; / Cytochrome P450 4F2; / Cytochrome P450-LTB-omega; / Leukotriene-B; / 4; / 20-monooxygenase 1
SpeciesHuman
Gene ID8529
UniprotP78329
Species.1
Gene ID.1
Uniprot.1
Species.2
Gene ID.2
Uniprot.2
Cellular LocalizationMicrosome membrane; Peripheral membrane protein . Endoplasmic reticulum membrane; Peripheral membrane protein .
Tissue ExpressionLiver. Also present in kidney: specifically expressed in the S2 and S3 segments of proximal tubules in cortex and outer medulla (PubMed:10660572).
Signaling_pathway
Research Areas>>Arachidonic acid metabolism; / >>Metabolic pathways
FunctionCatalytic activity:(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate + NADPH + O(2) = (6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + NADP(+) + H(2)O.,cofactor:Heme group.,Function:Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.,similarity:Belongs to the cytochrome P450 family.,tissue specificity:Liver.,
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