Name: Cytokeratin 5 [ABT051] mouse mAb
SKU: WM1475
Price: 268 USD
Availability: InStock

Product details

Background:
CK5 is a cytokeratin with a molecular weight of 58 kDa. In normal tissues, CK5 is expressed in squamous epithelium, germinal layer cells of some squamous epithelium, basal cells of ductal epithelium, myoepithelial cells and mesothelial cells, and almost no expression in most glandular epithelial cells. Therefore, it can be used for the differential diagnosis of squamous cell carcinoma and adenocarcinoma, mesothelioma and adenocarcinoma, as well as benign and malignant tumors of prostate, breast and salivary gland epithelium.

Specifications

Size	50μL/100μL
Target	Cytokeratin 5
Reactivity	Human, Dog
Applications	IHC, WB, IF, ELISA
MW(Calculated)	62kD
MW(Observed)	62kD
Host Species	Mouse
Isotype	IgG2a,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:200-1000; WB 1:500-2000; IF 1:100-500; ELISA 1:1000-5000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein G affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RMM051
Immunogen	Synthesized peptide derived from human Cytokeratin 5 AA range: 500-590
Specificity	The antibody can specifically recognize human CK5 protein, and shows no cross reaction with CK6. In western blotting of A431, A549 and Hela cell lysates, the antibody can label a 58 kDa band corresponding to CK5 only in A431 cell lysates.
Gene Name	KRT5
Protein Name	Keratin, type II cytoskeletal 5 (58 kDa cytokeratin) (Cytokeratin-5) (CK-5) (Keratin-5) (K5) (Type-II keratin Kb5)
Other Name	58 kDa cytokeRatin; / CK-5; / CK5; / CytokeRatin-5; / CytokeRatin5; / DDD; / DDD1; / EBS2; / epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; / K2C5_HUMAN; / K5; / keRatin 5; / epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types; / KeRatin 5; / KeRatin; / keRatin complex 2, basic, gene 5; / keRatin, type II cytoskeletal 5; / KeRatin-5; / KeRatin5; / KRT 5; / Krt5; / KRT5A; / type II cytoskeletal 5; / Type-II keRatin Kb5
Species	Human
Gene ID	3852
Uniprot	P13647
Species.1
Gene ID.1
Uniprot.1
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Cytoplasmic, Membranous
Tissue Expression	Expressed in corneal epithelium (at protein level).
Signaling_pathway
Research Areas
Function	Disease:Defects in KRT5 are a cause of epidermolysis bullosa simplex Dowling-Meara type (DM-EBS) [MIM:131760]. DM-EBS is a severe form of intraepidermal epidermolysis bullosa characterized by generalized herpetiform blistering, milia formation, dystrophic nails, and mucous membrane involvement.,Disease:Defects in KRT5 are a cause of epidermolysis bullosa simplex Koebner type (K-EBS) [MIM:131900]. K-EBS is a form of intraepidermal epidermolysis bullosa characterized by generalized skin blistering. The phenotype is not fundamentally distinct from the Dowling-Meara type, althought it is less severe.,Disease:Defects in KRT5 are a cause of epidermolysis bullosa simplex Weber-Cockayne type (WC-EBS) [MIM:131800]. WC-EBS is a form of intraepidermal epidermolysis bullosa characterized by blistering limited to palmar and plantar areas of the skin.,Disease:Defects in KRT5 are the cause of Dowling-Degos disease (DDD) [MIM:179850]; also known as Dowling-Degos-Kitamura disease or reticulate acropigmentation of Kitamura. DDD is an autosomal dominant genodermatosis. Affected individuals develop a postpubertal reticulate hyperpigmentation that is progressive and disfiguring, and small hyperkeratotic dark brown papules that affect mainly the flexures and great skin folds. Patients usually show no abnormalities of the hair or nails.,Disease:Defects in KRT5 are the cause of epidermolysis bullosa simplex with migratory circinate erythema (EBSMCE) [MIM:609352]. EBSMCE is a form of intraepidermal epidermolysis bullosa characterized by unusual migratory circinate erythema. Skin lesions appear from birth primarily on the hands, feet, and legs but spare nails, ocular epithelia and mucosae. Lesions heal with brown pigmentation but no scarring. Electron microscopy findings are distinct from those seen in the DM-EBS, with no evidence of tonofilament clumping.,Disease:Defects in KRT5 are the cause of epidermolysis bullosa simplex with mottled pigmentation (MP-EBS) [MIM:131960]. MP-EBS is a form of intraepidermal epidermolysis bullosa characterized by blistering at acral sites and 'mottled' pigmentation of the trunk and proximal extremities with hyper- and hypopigmentation macules.,miscellaneous:There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).,similarity:Belongs to the intermediate filament family.,subunit:Heterotetramer of two type I and two type II keratins. Keratin-5 associates with keratin-14. Interacts with TCHP.,

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Cytokeratin 5 [ABT051] mouse mAb

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Specifications

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Cytokeratin 5 [ABT051] mouse mAb

Product details

Specifications

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