Cytokeratin 7 Polyclonal Antibody
Ucallm_WP11939-50
Price range: $268.00 through $328.00
Product details
Background:
keratin 7(KRT7) Homo sapiens The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | Cytokeratin 7 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 51kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:40000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Keratin 7. AA range:420-469 |
| Specificity | Cytokeratin 7 Polyclonal Antibody detects endogenous levels of Cytokeratin 7 protein. |
| Gene Name | KRT7 SCL |
| Protein Name | Keratin type II cytoskeletal 7 |
| Other Name | KRT7; / SCL; / Keratin; / type II cytoskeletal 7; / Cytokeratin-7; / CK-7; / Keratin-7; / K7; / Sarcolectin; / Type-II keratin Kb7 |
| Species | Human |
| Gene ID | 3855 |
| Uniprot | P08729, |
| Species.1 | Mouse |
| Gene ID.1 | 110310 |
| Uniprot.1 | Q9DCV7, |
| Species.2 | Rat |
| Gene ID.2 | 300242 |
| Uniprot.2 | Q6IG12 |
| Cellular Localization | Cytoplasm . |
| Tissue Expression | Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus. |
| Signaling_pathway | |
| Research Areas | |
| Function | Function:Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).,induction:Up-regulated by retinoic acid.,mass spectrometry: PubMed:11840567,miscellaneous:There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).,PTM:Arg-20 is dimethylated, probably to asymmetric dimethylarginine.,similarity:Belongs to the intermediate filament family.,subunit:Heterotetramer of two type I and two type II keratins. Interacts with eukaryotic translation initiator factor 3 (eIF3) subunit EIF3S10 and with HPV16 E7.,tissue specificity:Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus., |
Please login to post questions
Related products
