Cytokeratin 8&18 [ABT053/CK18] mouse mAb
Ucallm_WM1402-50
Price range: $268.00 through $328.00
Product details
Background:
Cytokeratin 18 (CK18), an intermediated filament protein with a molecular weight of 45 KD, belongs to type I cytokeratin family. Cytokeratin 8 (CK8) is an intermediated filament protein with a molecular weight of 52 KD. It is a member of cytokeratin type II family. They are widely expressed in monolayer epithelial cells of thyroid, breast, gastrointestinal tract and respiratory tract. Ck8/18 is often used as a useful marker to identify adenocarcinoma from simple epithelial cells.
Specifications
| Size | 50μL/100μL |
| Target | Cytokeratin 8/18 |
| Reactivity | Human, Mouse, Rat |
| Applications | IHC, WB, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 48,54kD |
| Host Species | Mouse |
| Isotype | IgG2b,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1000; WB 1:500-2000; IF 1:100-500; ELISA 1:1000-5000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein G affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RMM053/CK18 |
| Immunogen | Synthesized peptide derived from human Cytokeratin 8&18 AA range: C-term to CK8 and 200-300 to CK18 |
| Specificity | This product can specifically recognize human CK8 and CK18 protein. It is a cocktail of anti-CK8 and anti-CK18 monoclonal antibodies. |
| Gene Name | KRT8/KRT18 |
| Protein Name | |
| Other Name | CARD2; / CK 8; / CK-8; / CK8; / CYK8; / CYKER; / Cytokeratin endo A; / CytokeRatin-8; / DreK8; / EndoA; / K2C8; / K2C8_HUMAN; / K8; / KeRatin 8; / KeRatin type II cytoskeletal 8; / KeRatin, type II cytoskeletal 8; / KeRatin-8; / KO; / Krt 2.8; / KRT8; / MGC118110; / MGC174782; / MGC53564; / MGC85764; / sb:cb186; / Type-II keRatin Kb8; / CK 18; / CK-18; / CK18; / CYK18; / Cytokeratin 18; / Cytokeratin endo B; / CytokeRatin-18; / K 18; / K18; / K1C18_HUMAN; / KA18; / KeRatin 18; / KeRatin 18, type I; / KeRatin D; / keRatin, type I cytoskeletal 18; / KeRatin-18; / Krt18 |
| Species | Human |
| Gene ID | 3856;3875 |
| Uniprot | P05787;P05783 |
| Species.1 | |
| Gene ID.1 | |
| Uniprot.1 | |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasmic, Membranous |
| Tissue Expression | Liver/ Tonsil |
| Signaling_pathway | Organismal Systems >> Endocrine system >> Estrogen signaling pathway |
| Research Areas | |
| Function | Disease:Defects in KRT8 are a cause of cryptogenic cirrhosis [MIM:215600].,Function:Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.,miscellaneous:There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).,PTM:O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.,PTM:Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.,similarity:Belongs to the intermediate filament family.,subunit:Heterotetramer of two type I and two type II keratins. keratin-8 associates with keratin-18. Associates with KRT20. Interacts with HCV core protein and PNN. When associated with KRT19, interacts with DMD. Interacts with TCHP.,tissue specificity:Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity., |
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