Price range: $268.00 through $328.00
Background:
Death-associated protein kinase 3 (DAPK3) induces morphological changes in apoptosis when overexpressed in mammalian cells. These results suggest that DAPK3 may play a role in the induction of apoptosis. [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | DAPK3 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 52kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human DAPK3. AA range:241-290 |
| Specificity | DAPK3 Polyclonal Antibody detects endogenous levels of DAPK3 protein. |
| Gene Name | DAPK3 |
| Protein Name | Death-associated protein kinase 3 |
| Other Name | DAPK3; / ZIPK; / Death-associated protein kinase 3; / DAP kinase 3; / DAP-like kinase; / Dlk; / MYPT1 kinase; / Zipper-interacting protein kinase; / ZIP-kinase |
| Species | Human |
| Gene ID | 1613 |
| Uniprot | O43293, |
| Species.1 | Mouse |
| Gene ID.1 | 13144 |
| Uniprot.1 | O54784, |
| Species.2 | Rat |
| Gene ID.2 | 64391 |
| Uniprot.2 | O88764 |
| Cellular Localization | Nucleus . Cytoplasm . Predominantly localizes to the cytoplasm but can shuttle between the nucleus and cytoplasm; cytoplasmic localization is promoted by phosphorylation at Thr-299 and involves Rho/Rock signaling. .; [Isoform 1]: Nucleus . Cytoplasm .; [Isoform 2]: Nucleus . Cytoplasm . |
| Tissue Expression | Widely expressed. Isoform 1 and isoform 2 are expressed in the bladder smooth muscle. |
| Signaling_pathway | Cellular Processes >> Transport and catabolism >> Autophagy - animal;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: specific types >> Bladder cancer |
| Research Areas | >>Autophagy - animal; / >>Pathways in cancer; / >>Bladder cancer |
| Function | Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,Function:Serine/threonine kinase which acts as a positive regulator of apoptosis. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.,similarity:Contains 1 protein kinase domain.,subcellular location:Relocates to the cytoplasm on binding PAWR where the complex appears to interact with actin filaments (By similarity). Associates to centromeres from prophase to anaphase.,subunit:Homodimer or forms heterodimers with ATF4. Both interactions require an intact leucine zipper domain and oligomerization is required for full enzymatic activity. Also binds to DAXX and PAWR, possibly in a ternary complex which plays a role in caspase activation. Interacts with AATF and CDC5L., |
