U-Blot® Dnmt3a Rabbit mAb
WR4691 Western Blot analysis using Rat brain lysates,primary antibody was diluted at 1:1000, 4°over night, Dylight 800 secondary antibody(WS0446)was diluted at 1:10000, 37° 1hour.
Price range: $268.00 through $328.00
Product details
Background:CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. [provided by RefSeq, Mar 2016],
Specifications
| Size | 50μL/100μL |
| Target | DNM3A |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 102kD |
| MW(Observed) | 130kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:100-1:500; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1011R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | DNMT3A |
| Protein Name | DNA (cytosine-5)-methyltransferase 3A (Dnmt3a) (DNA methyltransferase HsaIIIA) (DNA MTase HsaIIIA) (M.HsaIIIA) |
| Other Name | |
| Species | Human |
| Gene ID | 1788 |
| Uniprot | Q9Y6K1, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | O88508, |
| Species.2 | Rat |
| Gene ID.2 | |
| Uniprot.2 | Q1LZ53 |
| Cellular Localization | Nucleus . Chromosome . Cytoplasm . Accumulates in the major satellite repeats at pericentric heterochromatin. . |
| Tissue Expression | Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung. |
| Signaling_pathway | Human Diseases >> Cancer: overview >> MicroRNAs in cancer |
| Research Areas | >>Cysteine and methionine metabolism; / >>Metabolic pathways; / >>MicroRNAs in cancer |
| Function | Catalytic activity:S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.,Caution:It is uncertain whether Met-1 or Met-4 is the initiator.,Function:Required for genome wide de novo methylation and is essential for development. DNA methylation is coordinated with methylation of histones.,similarity:Belongs to the C5-methyltransferase family.,similarity:Contains 1 ADD-type zinc finger.,similarity:Contains 1 PWWP domain.,subunit:Binds the ZNF238 transcriptional repressor. Interacts with SETDB1. Associates with HDAC1 through its ADD-type zinc-finger (By similarity). Interacts with DNMT1 and DNMT3B. Interacts with the PRC2/EED-EZH2 complex.,tissue specificity:Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung., |
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